Gan Han Ming, Gan Huan You, Tan Mun Hua, Penny Shane S, Willan Richard C, Austin Christopher M
a School of Science, Monash University Malaysia, Jalan Lagoon Selatan , Petaling Jaya , Selangor , Malaysia .
b Genomics Facility, Monash University Malaysia, Jalan Lagoon Selatan , Petaling Jaya , Selangor , Malaysia .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3220-1. doi: 10.3109/19401736.2015.1007355. Epub 2015 Feb 4.
The complete mitochondrial genome of the commercially and ecologically important and internationally vulnerable giant clam Tridacna squamosa was recovered by genome skimming using the MiSeq platform. The T. squamosa mitogenome has 20,930 base pairs (62.35% A+T content) and is made up of 12 protein-coding genes, 2 ribosomal subunit genes, 24 transfer RNAs, and a 2594 bp non-coding AT-rich region. The mitogenome has a relatively large insertion in the atp6 gene. This is the first mitogenome to be sequenced from the genus Tridacna, and the family Tridacnidae and represents a new gene order.
利用MiSeq平台通过基因组鸟枪法测序获得了具有商业和生态重要性且在国际上处于易危状态的大砗磲(Tridacna squamosa)的完整线粒体基因组。大砗磲的线粒体基因组有20,930个碱基对(A+T含量为62.35%),由12个蛋白质编码基因、2个核糖体亚基基因、24个转运RNA和一个2594 bp的富含AT的非编码区域组成。该线粒体基因组在atp6基因中有一个相对较大的插入。这是首个从砗磲属以及砗磲科测序得到的线粒体基因组,代表了一种新的基因排列顺序。