Dolu Nazan, Acer Hale, Kara Ali Yucel
Erciyes University, Faculty of Medicine, Depart. of Physiology, Kayseri, Turkey.
Erciyes University, Institute of Health Sciences, Department of Physiology, Kayseri, Turkey.
Acta Medica (Hradec Kralove). 2014;57(3):112-8. doi: 10.14712/18059694.2014.49.
Carnosine is a dipeptide formed of the amino acids β-alanine and histidine. Only a limited number of studies have examined the effects of carnosine on sympathetic nerve activation and anxiety. The present study was undertaken to determine the dose-related effects of carnosine on anxiety in the elevated T-maze test (ETM) with electrodermal activity (EDA). Carnosine was injected in three groups of rats with doses of 10 (low dose), 100 (medium dose) and 1000 (high dose) mg/kg i.p. Physiological saline was injected in the sham group. The anxiety scores of the rats were measured with ETM 20 minutes after injection. Then, SCL was measured. The decreased number of entries into the open arm (NEOA), the percentage of time spent in the open arm (% TSOA) and higher EDA [shown by skin conductance level (SCL)] indicate higher anxiety. The NEOA and % TSOA were lower in the high-dose group than in the other groups. SCL was lower in the medium-dose carnosine group than in the high-dose carnosine and sham groups. SCL was higher in the high-dose group than in the medium-dose and sham groups. Our results suggest that high-dose carnosine produced anxiety-like effects as assessed in the SCL and ETM. Medium-dose carnosine acted as an anxiolytic. The anxiety-related responses of carnosine depend on its dose-related effect.
肌肽是一种由β-丙氨酸和组氨酸组成的二肽。仅有有限的一些研究考察了肌肽对交感神经激活和焦虑的影响。本研究旨在通过皮电活动（EDA）在高架T迷宫试验（ETM）中确定肌肽对焦虑的剂量相关效应。将肌肽以10（低剂量）、100（中剂量）和1000（高剂量）mg/kg腹腔注射给三组大鼠。假手术组注射生理盐水。注射后20分钟用ETM测量大鼠的焦虑评分。然后，测量皮电水平（SCL）。进入开放臂的次数减少（NEOA）、在开放臂中花费的时间百分比（%TSOA）以及更高的EDA[以皮电水平（SCL）表示]表明焦虑程度更高。高剂量组的NEOA和%TSOA低于其他组。中剂量肌肽组的SCL低于高剂量肌肽组和假手术组。高剂量组的SCL高于中剂量组和假手术组。我们的结果表明，如在SCL和ETM中所评估的，高剂量肌肽产生了类似焦虑的效应。中剂量肌肽起到了抗焦虑作用。肌肽与焦虑相关的反应取决于其剂量相关效应。
