Ra Chae Hun, Jung Jang Hyun, Sunwoo In Young, Kang Chang Han, Jeong Gwi-Taek, Kim Sung-Koo
Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.
J Microbiol Biotechnol. 2015 Jun;25(6):856-62. doi: 10.4014/jmb.1409.09038.
The objective of this study was to optimize the slurry contents and salt concentrations for ethanol production from hydrolysates of the seaweed Eucheuma spinosum. A monosaccharide concentration of 44.2 g/l as 49.6% conversion of total carbohydrate of 89.1 g/l was obtained from 120 g dw/l seaweed slurry. Monosaccharides from E. spinosum slurry were obtained by thermal acid hydrolysis and enzymatic hydrolysis. Addition of activated carbon at 2.5% (w/v) and the adsorption time of 2 min were used in subsequent adsorption treatments to prevent the inhibitory effect of HMF. The adsorption surface area of the activated carbon powder was 1,400-1,600 m(2)/g and showed selectivity to 5-hydroxymethyl furfural (HMF) from monosaccharides. Candida tropicalis KCTC 7212 was cultured in yeast extract, peptone, glucose, and high-salt medium, and exposed to 80, 90, 100, and 110 practical salinity unit (psu) salt concentrations in the lysates. The 100 psu salt concentration showed maximum cell growth and ethanol production. The ethanol fermentations with activated carbon treatment and use of C. tropicalis acclimated to a high salt concentration of 100 psu produced 17.9 g/l of ethanol with a yield (YEtOH) of 0.40 from E. spinosum seaweed.
本研究的目的是优化从多刺麒麟菜水解物生产乙醇的浆液含量和盐浓度。从120 g干重/升的海藻浆液中获得了44.2 g/升的单糖浓度，占89.1 g/升总碳水化合物的49.6%转化率。多刺麒麟菜浆液中的单糖通过热酸水解和酶水解获得。在随后的吸附处理中使用2.5%（w/v）的活性炭添加量和2分钟的吸附时间，以防止5-羟甲基糠醛（HMF）的抑制作用。活性炭粉末的吸附表面积为1400 - 1600 m²/g，对单糖中的5-羟甲基糠醛（HMF）具有选择性。热带假丝酵母KCTC 7212在酵母提取物、蛋白胨、葡萄糖和高盐培养基中培养，并在裂解物中暴露于80、90、100和110实际盐度单位（psu）的盐浓度下。100 psu的盐浓度显示出最大的细胞生长和乙醇产量。采用活性炭处理以及使用适应100 psu高盐浓度的热带假丝酵母进行乙醇发酵，从多刺麒麟菜海藻中产生了17.9 g/升的乙醇，产率（YEtOH）为0.40。
