Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
J Microbiol Biotechnol. 2020 Jan 28;30(6):930-936. doi: 10.4014/jmb.2002.02014.
The red seaweed has been used for the production of bioethanol. Pretreatment for monosaccharide production was carried out with 12% (w/v) slurry and 500 mM HNO at 121°C for 90 min. Enzymatic hydrolysis was performed with a mixture of commercial enzymes (Cellic C-Tec 2 and Celluclast 1.5 L; 16 U/ml) at 50°C and 150 rpm for 48 h. was composed of 66.9% carbohydrates. In this study, 61.0 g/L monosaccharides were obtained from 120.0 g dw/l . The fermentation inhibitors such as hydroxymethylfurfural (HMF), levulinic acid, and formic acid were produced during pretreatment. Activated carbon was used to remove HMF. Wildtype and adaptively evolved , , and were used for fermentation to evaluate ethanol production.
红海藻已被用于生产生物乙醇。采用 12%（w/v）料浆和 500 mM HNO 在 121°C 下预处理 90 分钟以生产单糖。在 50°C 和 150 rpm 下用混合商业酶（Cellic C-Tec 2 和 Celluclast 1.5 L；16 U/ml）进行酶解 48 小时。干重中含有 66.9%的碳水化合物。在这项研究中，从 120.0 g dw/l 的红海藻中获得了 61.0 g/L 的单糖。在预处理过程中会产生羟甲基糠醛（HMF）、乙酰丙酸和甲酸等发酵抑制剂。使用活性炭去除 HMF。野生型和适应性进化的 、 、 和 用于发酵以评估乙醇生产。
