Ra Chae Hun, Nguyen Trung Hau, Jeong Gwi-Taek, Kim Sung-Koo
Department of Biotechnology, Pukyong National University, Busan 48513, South Korea.
Department of Biotechnology, Pukyong National University, Busan 48513, South Korea.
Bioresour Technol. 2016 Jun;209:66-72. doi: 10.1016/j.biortech.2016.02.106. Epub 2016 Mar 3.
Hyper thermal (HT) acid hydrolysis of Kappaphycus alvarezii, a red seaweed, was optimized to 12% (w/v) seaweed slurry content, 180mM H2SO4 at 140°C for 5min. The maximum monosaccharide concentration of 38.3g/L and 66.7% conversion from total fermentable monosaccharides of 57.6g/L with 120gdw/L K. alvarezii slurry were obtained from HT acid hydrolysis and enzymatic saccharification. HT acid hydrolysis at a severity factor of 0.78 efficiently converted the carbohydrates of seaweed to monosaccharides and produced a low concentration of inhibitory compounds. The levels of ethanol production by separate hydrolysis and fermentation with non-adapted and adapted Kluyveromyces marxianus to high concentration of galactose were 6.1g/L with ethanol yield (YEtOH) of 0.19 at 84h and 16.0g/L with YEtOH of 0.42 at 72h, respectively. Development of the HT acid hydrolysis process and adapted yeast could enhance the overall ethanol fermentation yields of K. alvarezii seaweed.
对红藻卡帕藻进行高温（HT）酸水解的工艺进行了优化，海藻浆含量为12%（w/v），硫酸浓度为180mM，在140°C下反应5分钟。在120gdw/L卡帕藻浆的情况下，通过高温酸水解和酶糖化反应，可获得最大单糖浓度为38.3g/L，总可发酵单糖57.6g/L的转化率为66.7%。高温酸水解在强度因子为0.78时能有效地将海藻碳水化合物转化为单糖，并产生低浓度的抑制性化合物。使用未适应和适应高浓度半乳糖的马克斯克鲁维酵母进行单独水解和发酵时，乙醇产量分别为84小时时6.1g/L，乙醇产率（YEtOH）为0.19；72小时时16.0g/L，YEtOH为0.42。高温酸水解工艺和适应性酵母的开发可以提高卡帕藻海藻的整体乙醇发酵产率。
