Tong Xiaoli, Guo Na, Dang Zhiyan, Ren Qingguang, Shen Hao
Center of Analysis and Measurement, Fudan University, Shanghai, People's Republic of China.
Modern Physics Research Center, Fudan University, Shanghai, People's Republic of China.
IET Nanobiotechnol. 2018 Oct;12(7):987-993. doi: 10.1049/iet-nbt.2017.0230.
Nanoparticles (NPs), especially biosynthesised in living plants by absorbing soluble salts and reducing metal ions, are extensively used in various fields. This work aimed at investigating the in vivo biosynthesis of silver NPs (Ag-NPs) in maize and the spatial distribution of the NPs and some important nutrient elements in the plant. The content of silver in plant was examined by inductively coupled plasma-atomic emission spectrometer showing that Ag can be absorbed by plant as soluble salts. The NPs in different parts of maize plant were detected and analysed by transmission electron microscopy, demonstrating the synthesis of NPs and their transport from the root to the shoots. Two-dimensional proton induced X-ray emission of silver, chlorine and several nutrient elements elucidated the possible relationship between synthesis of NPs and several nutrient elements in plant tissues. To their knowledge, this is the first report of possibility of synthesis of Ag-NPs in living plants maize ( L.). This study presents direct evidence for synthesis of NPs and distribution of related nutrient elements in maize, which has great significance for studying synthetic application of NPs in crop plants.
纳米颗粒（NPs），特别是通过吸收可溶性盐和还原金属离子在活植物中生物合成的纳米颗粒，被广泛应用于各个领域。这项工作旨在研究玉米中银纳米颗粒（Ag-NPs）的体内生物合成以及植物中纳米颗粒和一些重要营养元素的空间分布。通过电感耦合等离子体原子发射光谱仪检测植物中的银含量，结果表明银可以作为可溶性盐被植物吸收。利用透射电子显微镜对玉米植株不同部位的纳米颗粒进行了检测和分析，证实了纳米颗粒的合成及其从根部到地上部分的运输。对银、氯和几种营养元素的二维质子诱导X射线发射分析揭示了植物组织中纳米颗粒合成与几种营养元素之间的可能关系。据他们所知，这是关于活植物玉米（L.）中合成Ag-NPs可能性的首次报道。本研究为玉米中纳米颗粒的合成及相关营养元素的分布提供了直接证据，这对于研究纳米颗粒在作物中的合成应用具有重要意义。
