Li Feifei, Burgie E Sethe, Yu Tao, Héroux Annie, Schatz George C, Vierstra Richard D, Orville Allen M
Photon Sciences Directorate and ∥Biosciences Department, Brookhaven National Laboratory , Upton, New York 11973, United States.
J Am Chem Soc. 2015 Mar 4;137(8):2792-5. doi: 10.1021/ja510923m. Epub 2015 Feb 18.
We report that in the red light-absorbing (Pr) state, the bilin chromophore of the Deinococcus radiodurans proteobacterial phytochrome (DrBphP) is hypersensitive to X-ray photons used in typical synchrotron X-ray protein crystallography experiments. This causes the otherwise fully protonated chromophore to deprotonate without additional major structural changes. These results have major implications for our understanding of the structural and chemical characteristics of the resting and intermediate states of phytochromes and other photoreceptor proteins.
我们报告称，在吸收红光（Pr）状态下，耐辐射球菌（Deinococcus radiodurans）的变形菌门植物色素（DrBphP）中的胆色素发色团对典型同步加速器X射线蛋白质晶体学实验中使用的X射线光子高度敏感。这导致原本完全质子化的发色团去质子化，而没有额外没有其他主要结构变化。这些结果对我们理解植物色素和其他光感受器蛋白的静止和中间状态的结构和化学特征具有重要意义。
