Lu Hoang D, Spiegel Alina C, Hurley Amanda, Perez Lark J, Maisel Katharina, Ensign Laura M, Hanes Justin, Bassler Bonnie L, Semmelhack Martin F, Prud'homme Robert K
∥Department of Chemistry and Biochemistry, Rowan University, Glassboro, New Jersey 08028, United States.
○Howard Hughes Medical Institute, Chevy Chase, Maryland 20815, United States.
Nano Lett. 2015 Apr 8;15(4):2235-41. doi: 10.1021/acs.nanolett.5b00151. Epub 2015 Mar 3.
The rise of bacterial antibiotic resistance has created a demand for alternatives to traditional antibiotics. Attractive possibilities include pro- and anti-quorum sensing therapies that function by modulating bacterial chemical communication circuits. We report the use of Flash NanoPrecipitation to deliver the Vibrio cholerae quorum-sensing signal CAI-1 ((S)-3-hydroxytridecan-4-one) in a water dispersible form as nanoparticles. The particles activate V. cholerae quorum-sensing responses 5 orders of magnitude higher than does the identically administered free CAI-1 and are diffusive across in vivo delivery barriers such as intestinal mucus. This work highlights the promise of combining quorum-sensing strategies with drug delivery approaches for the development of next-generation medicines.
细菌对抗生素耐药性的增加引发了对传统抗生素替代品的需求。有吸引力的可能性包括通过调节细菌化学通讯回路起作用的群体感应促进和群体感应抑制疗法。我们报告了使用快速纳米沉淀法将霍乱弧菌群体感应信号CAI-1((S)-3-羟基十三烷-4-酮)以纳米颗粒的形式制成水分散体。这些颗粒激活霍乱弧菌群体感应反应的能力比同等剂量的游离CAI-1高5个数量级,并且能够扩散穿过体内递送屏障,如肠道黏液。这项工作突出了将群体感应策略与药物递送方法相结合以开发下一代药物的前景。