Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA.
Nat Nanotechnol. 2015 Feb;10(2):176-82. doi: 10.1038/nnano.2014.282.
Label and label-free methods to image carbon-based nanomaterials exist. However, label-based approaches are limited by the risk of tag detachment over time, and label-free spectroscopic methods have slow imaging speeds, weak photoluminescence signals and strong backgrounds. Here, we present a label-free mass spectrometry imaging method to detect carbon nanotubes, graphene oxide and carbon nanodots in mice. The large molecular weights of nanoparticles are difficult to detect using conventional mass spectrometers, but our method overcomes this problem by using the intrinsic carbon cluster fingerprint signal of the nanomaterials. We mapped and quantified the sub-organ distribution of the nanomaterials in mice. Our results showed that most carbon nanotubes and nanodots were found in the outer parenchyma of the kidney, and all three materials were seen in the red pulp of the spleen. The highest concentrations of nanotubes in the spleen were found within the marginal zone.
存在用于对基于碳的纳米材料进行成像的标记和无标记方法。然而，基于标记的方法受到标记随着时间的推移而脱落的风险的限制，而无标记的光谱方法则具有成像速度慢、光致发光信号弱和背景强的缺点。在这里，我们提出了一种无标记质谱成像方法来检测小鼠中的碳纳米管、氧化石墨烯和碳纳米点。使用传统的质谱仪很难检测到纳米颗粒的大分子量，但我们的方法通过利用纳米材料的固有碳簇指纹信号克服了这个问题。我们对纳米材料在小鼠中的亚器官分布进行了定位和定量。我们的结果表明，大多数碳纳米管和纳米点存在于肾脏的外实质中，而三种材料均存在于脾脏的红髓中。在脾脏中，纳米管的浓度最高的区域位于边缘区。
