• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于活海马组织切片大气压质谱成像的纳米材料与连续波激光高效解吸

Nanomaterials and continuous wave laser-based efficient desorption for atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices.

作者信息

Kim Jae Young, Seo Eun Seok, Lim Hee Jin, Kim Hyunmin, Park Ji-Won, Shin Hyeon Ho, Lim Dong-Kwon, Moon Dae Won

机构信息

Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST) Daegu Republic of Korea

Companion Diagnostics and Medical Technology Research Group, Daegu Gyeongbuk Institute of Science and Technology (DGIST) Daegu Republic of Korea.

出版信息

RSC Adv. 2018 Feb 20;8(15):8021-8025. doi: 10.1039/c8ra00038g. eCollection 2018 Feb 19.

DOI:10.1039/c8ra00038g
PMID:35541999
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9078475/
Abstract

Micrometer-resolution mass spectrometric imaging of live hippocampal tissue is achieved with a highly efficient desorption of biomolecules using a 532 nm continuous wave laser and gold nanoparticles or graphene oxide as an energy transporter, which enables clear identification of the distributions of monoacylglycerol, adenine, cholesterol, sphingosine and ceramide.

摘要

利用532纳米连续波激光以及金纳米颗粒或氧化石墨烯作为能量传输体，对生物分子进行高效解吸，实现了对活海马组织的微米级分辨率质谱成像，这使得能够清晰识别单酰甘油、腺嘌呤、胆固醇、鞘氨醇和神经酰胺的分布。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/691b28df4700/c8ra00038g-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/8e09a70236b2/c8ra00038g-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/781994059b4a/c8ra00038g-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/9257641b61a1/c8ra00038g-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/df73757f90ce/c8ra00038g-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/15e8d572d02e/c8ra00038g-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/691b28df4700/c8ra00038g-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/8e09a70236b2/c8ra00038g-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/781994059b4a/c8ra00038g-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/9257641b61a1/c8ra00038g-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/df73757f90ce/c8ra00038g-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/15e8d572d02e/c8ra00038g-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b7f8/9078475/691b28df4700/c8ra00038g-f5.jpg

相似文献

1
Nanomaterials and continuous wave laser-based efficient desorption for atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices.用于活海马组织切片大气压质谱成像的纳米材料与连续波激光高效解吸
RSC Adv. 2018 Feb 20;8(15):8021-8025. doi: 10.1039/c8ra00038g. eCollection 2018 Feb 19.
2
Graphene-Coated Glass Substrate for Continuous Wave Laser Desorption and Atmospheric Pressure Mass Spectrometric Imaging of a Live Hippocampal Tissue.基于石墨烯涂层玻璃基底的连续波激光解吸常压质谱成像技术对活体海马组织的分析
ACS Appl Mater Interfaces. 2019 Jul 31;11(30):27153-27161. doi: 10.1021/acsami.9b02620. Epub 2019 Jun 22.
3
Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution.大气压质谱成像技术对活海马体组织切片进行亚细胞空间分辨率分析。
Nat Commun. 2017 Dec 13;8(1):2113. doi: 10.1038/s41467-017-02216-6.
4
Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues.环境质谱成像技术在细胞和组织中小分子的应用。
Methods Mol Biol. 2022;2437:41-59. doi: 10.1007/978-1-0716-2030-4_3.
5
Atmospheric pressure mass spectrometric imaging of bio-tissue specimen using electrospray-assisted CW laser desorption and ionization source.利用电喷雾辅助连续波激光解吸电离源的大气压质谱成像技术对生物组织样本进行分析。
Biointerphases. 2019 Jul 26;14(4):041001. doi: 10.1116/1.5099563.
6
Surface analysis of lipids by mass spectrometry: more than just imaging.质谱法分析脂质的表面特性：不止于成像。
Prog Lipid Res. 2013 Oct;52(4):329-53. doi: 10.1016/j.plipres.2013.04.005. Epub 2013 Apr 24.
7
A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule.一种使用磷酸盐捕获分子对血液和实体组织中的鞘氨醇-1-磷酸和神经酰胺-1-磷酸进行质谱分析的净化方法。
Methods Mol Biol. 2018;1697:57-71. doi: 10.1007/7651_2017_6.
8
Skeletal muscle fiber analysis by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometric imaging at high mass and high spatial resolution.通过大气压扫描微探针基质辅助激光解吸/电离质谱成像在高质量和高空间分辨率下进行骨骼肌纤维分析。
Proteomics. 2016 Jun;16(11-12):1822-4. doi: 10.1002/pmic.201500536.
9
Atmospheric pressure molecular imaging by infrared MALDI mass spectrometry.基于红外基质辅助激光解吸电离质谱的大气压分子成像
Anal Chem. 2007 Jan 15;79(2):523-32. doi: 10.1021/ac061577n.
10
Thin-layer chromatography combined with diode laser desorption/atmospheric pressure chemical ionization mass spectrometry.薄层色谱法与二极管激光解吸/大气压化学电离质谱联用
Rapid Commun Mass Spectrom. 2004;18(16):1803-8. doi: 10.1002/rcm.1556.

本文引用的文献

1
Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution.大气压质谱成像技术对活海马体组织切片进行亚细胞空间分辨率分析。
Nat Commun. 2017 Dec 13;8(1):2113. doi: 10.1038/s41467-017-02216-6.
2
Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques.使用直接液体萃取技术的常压质谱成像
Anal Chem. 2016 Jan 5;88(1):52-73. doi: 10.1021/acs.analchem.5b04188. Epub 2015 Nov 24.
3
The Structural Influence of Graphene Oxide on Its Fragmentation during Laser Desorption/Ionization Mass Spectrometry for Efficient Small-Molecule Analysis.
氧化石墨烯在激光解吸/电离质谱中用于高效小分子分析时其碎片化的结构影响
Chemistry. 2015 May 4;21(19):7217-23. doi: 10.1002/chem.201404067. Epub 2015 Mar 24.
4
Mass spectrometry imaging reveals the sub-organ distribution of carbon nanomaterials.质谱成像揭示了碳纳米材料的亚器官分布。
Nat Nanotechnol. 2015 Feb;10(2):176-82. doi: 10.1038/nnano.2014.282.
5
Ambient mass spectrometry imaging: plasma assisted laser desorption ionization mass spectrometry imaging and its applications.常压质谱成像：等离子体辅助激光解吸电离质谱成像及其应用
Anal Chem. 2014 May 6;86(9):4164-9. doi: 10.1021/ac403310k. Epub 2014 Apr 28.
6
Carbon nanodots as a matrix for the analysis of low-molecular-weight molecules in both positive- and negative-ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and quantification of glucose and uric acid in real samples.碳纳米点作为基质在正、负离子基质辅助激光解吸/电离飞行时间质谱分析中小分子的分析及实际样品中葡萄糖和尿酸的定量。
Anal Chem. 2013 Jul 16;85(14):6646-52. doi: 10.1021/ac401601r. Epub 2013 Jul 5.
7
Mass spectrometry imaging under ambient conditions.大气环境下的质谱成像。
Mass Spectrom Rev. 2013 May-Jun;32(3):218-43. doi: 10.1002/mas.21360. Epub 2012 Sep 20.
8
Mass spectral molecular networking of living microbial colonies.微生物菌落的质谱分子网络分析。
Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):E1743-52. doi: 10.1073/pnas.1203689109. Epub 2012 May 14.
9
Analysis of lipids with desorption atmospheric pressure photoionization-mass spectrometry (DAPPI-MS) and desorption electrospray ionization-mass spectrometry (DESI-MS).采用解吸常压光电离质谱（DAPPI-MS）和解吸电喷雾电离质谱（DESI-MS）分析脂质。
J Mass Spectrom. 2012 May;47(5):611-9. doi: 10.1002/jms.2992.
10
In situ, real-time identification of biological tissues by ultraviolet and infrared laser desorption ionization mass spectrometry.利用紫外和红外激光解吸电离质谱法对生物组织进行原位实时鉴定。
Anal Chem. 2011 Mar 1;83(5):1632-40. doi: 10.1021/ac102613m. Epub 2011 Feb 8.