Xiong Zhili, Wang Yanmin, Lang Lang, Ma Shuping, Zhao Longshan, Xiao Wei, Wang Yanjuan
School of Pharmacy, Shenyang Pharmaceutical University 103 Wenhua Road Shenyang 110016 China
National Key Laboratory of Pharmaceutical New Technology for Chinese Medicine, Jiangsu Kanion Pharmaceutical Co., Ltd 58 Haichang South Road, Xinpu District Lianyungang 222001 China.
RSC Adv. 2018 Mar 13;8(18):10023-10031. doi: 10.1039/c7ra13123b. eCollection 2018 Mar 5.
Acute lung injury (ALI) is a severe respiratory disease. To date, no medical interventions have been proven effective in improving the outcome. Reduning injection (RDN) showed a potential effect in the therapy of ALI. However, seldom does research concern the holistic pharmacological mechanisms of RDN on ALI. A metabolomic strategy, based on two consecutive extractions of the lung tissue, has been developed to investigate therapeutic mechanisms of RDN on ALI model rat. The extraction procedure was an aqueous extraction with methanol-water followed by organic extraction with dichloromethane-methanol. According to the lipophilicity of extracts, aqueous extracts were analyzed on the T3 column and organic extracts on the C18 column. Partial least-squares discriminant analysis was utilized to identify differences in metabolic profiles of rats. A total of 14 potential biomarkers in lung tissue were identified, which mainly related to phospholipid metabolism, sphingolipid metabolism, nucleotide metabolism and energy metabolism. The combined analytical method provides complementary metabolomics information for exploring the action mechanism of RDN against ALI. And the obtained results indicate metabolomics is a promising tool for understanding the holism and synergism of traditional Chinese medicine.
急性肺损伤（ALI）是一种严重的呼吸系统疾病。迄今为止，尚无医学干预措施被证明能有效改善其预后。热毒宁注射液（RDN）在ALI治疗中显示出潜在作用。然而，很少有研究关注RDN治疗ALI的整体药理机制。基于对肺组织进行连续两次提取，已开发出一种代谢组学策略来研究RDN对ALI模型大鼠的治疗机制。提取过程是先用甲醇-水进行水相提取，然后用二氯甲烷-甲醇进行有机相提取。根据提取物的亲脂性，水相提取物在T3柱上进行分析，有机相提取物在C18柱上进行分析。利用偏最小二乘判别分析来识别大鼠代谢谱的差异。共鉴定出肺组织中的14种潜在生物标志物，它们主要与磷脂代谢、鞘脂代谢、核苷酸代谢和能量代谢有关。该联合分析方法为探索RDN抗ALI的作用机制提供了互补的代谢组学信息。所得结果表明代谢组学是理解中药整体性和协同作用的一种有前景的工具。
