Hosseini-Esfahani Firoozeh, Mirmiran Parvin, Daneshpour Maryam S, Mehrabi Yadollah, Hedayati Mehdi, Soheilian-Khorzoghi Mona, Azizi Fereidoun
Nutrition and Endocrine Research Center, Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran,Iran.
Cellular Molecular and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran,Iran.
Br J Nutr. 2015 Feb 28;113(4):644-53. doi: 10.1017/S0007114514003687. Epub 2015 Feb 5.
The interaction of genetic and dietary factors, as an area of CVD research, has been explored poorly. The aim of the present study was to examine the interaction of dietary patterns and three genetic variants of APOA1 and APOC3, both independently and in combination, relative to the risk of the metabolic syndrome (MetS) in Tehranian adults. In the present matched, nested case-control study, 414 subjects with the MetS and 414 controls were selected from the participants of the Tehran Lipid and Glucose Study. Dietary patterns were determined by factor analysis. APOC3 (rs5128 3238C>G) and APOA1 (rs670, -75G>A and rs5069,+83C>T) SNP were genotyped by the conventional PCR followed by the restriction fragment length polymorphism technique. Overall, three major dietary patterns were extracted: healthy dietary pattern (HDP); Western dietary pattern (WDP); fat-sweet dietary pattern (FSDP). The A and T allele carriers of the APOA1 SNP had a greater risk of developing the MetS in the highest quartile of WDP scores (OR 3·22, 95 % CI 1·21, 8·58, P(interaction)= 0·03). Compared with other genotype combinations, the combined effect of APOC3/APOA1 (CC/GA+AA/CT+TT) genotypes showed a further increase in the risk of the MetS in the highest quartile of WDP scores (OR 1, 2·49, 8·73, 6·32, P trend< 0·001, P(interaction)= 0·003). A significant interaction was found between the quartiles of FSDP scores and the APOA1 diplotype (GA+AA/CT+TT). OR for these genotype carriers were 1, 0·65, 0·57 and 0·22 (P(trend)= 0·006) in the lowest to the highest quartile of FSDP scores when compared with the other combined genotypes (P(interaction)= 0·03). Our findings suggest that the WDP and FSDP are associated with APOA1 and APOC3 SNP in relation to the risk of the MetS.
作为心血管疾病（CVD）研究的一个领域，遗传因素与饮食因素的相互作用尚未得到充分探索。本研究的目的是考察饮食模式与APOA1和APOC3的三种基因变体单独及联合作用时，与德黑兰成年人代谢综合征（MetS）风险的相关性。在这项匹配的巢式病例对照研究中，从德黑兰脂质与葡萄糖研究的参与者中选取了414例患有MetS的受试者和414例对照。通过因子分析确定饮食模式。采用常规聚合酶链反应（PCR）及限制性片段长度多态性技术对APOC3（rs5128 3238C>G）和APOA1（rs670，-75G>A和rs5069，+83C>T）单核苷酸多态性（SNP）进行基因分型。总体而言，提取出三种主要饮食模式：健康饮食模式（HDP）；西方饮食模式（WDP）；高脂高糖饮食模式（FSDP）。在WDP得分最高四分位数组中，APOA1 SNP的A和T等位基因携带者发生MetS的风险更高（比值比[OR] 3.22，95%置信区间[CI] 1.21，8.58，交互作用P值=0.03）。与其他基因型组合相比，在WDP得分最高四分位数组中，APOC3/APOA1（CC/GA+AA/CT+TT）基因型的联合作用使MetS风险进一步增加（OR分别为1、2.49、8.73、6.32，趋势P值<0.001，交互作用P值=0.003）。在FSDP得分四分位数组与APOA1双倍型（GA+AA/CT+TT）之间发现显著交互作用。与其他联合基因型相比，在FSDP得分最低至最高四分位数组中，这些基因型携带者的OR分别为1、0.65、0.57和0.22（趋势P值=0.006）（交互作用P值=0.03）。我们的研究结果表明，就MetS风险而言，WDP和FSDP与APOA1和APOC3 SNP相关。
