Nasreddine Lara, Akika Reem, Mailhac Aurelie, Tamim Hani, Zgheib Nathalie Khoueiry
Department of Nutrition & Food Sciences, Faculty of Agriculture and Food Sciences, American University of Beirut, Beirut, PO Box 11-0236, Riad El-Solh 1107 2020, Lebanon.
Department of Pharmacology and Toxicology, Faculty of Medicine, American University of Beirut, Beirut, PO Box 11-0236, Riad El-Solh 1107 2020, Lebanon.
J Pers Med. 2019 Feb 5;9(1):11. doi: 10.3390/jpm9010011.
In contrast to the large number of genetic studies on obesity, there has been significantly less nutrigenetics investigation of the interaction between diet and single nucleotide polymorphisms (SNPs) in obesity, especially within Eastern Mediterranean populations. The aim of this study was to evaluate the potential interactions between three candidate SNPs, namely, and in the ( gene and the variant of the ( gene, and macronutrient intake with regard to obesity, body fat, and muscle composition. Three hundred and eight healthy Lebanese adults were included in this study. Data collection included a questionnaire for demographics and lifestyle in addition to a detailed dietary assessment using a culture-specific 80-item semi-quantitative food frequency questionnaire. This was coupled with anthropometric measurements and peripheral blood withdrawal for DNA and genotyping using Taqman allele discrimination assays. The two candidate SNPs were not associated with risk of obesity in this population sample, yet there was a trend, though not a significant one, towards lower muscle mass among carriers of the risk allele of either SNPs. To our knowledge, these results have not been previously reported. As for the variant, results were congruent with the literature, given that individuals who were homozygous for the risk allele had significantly higher body mass index (BMI) and body fat despite lower intakes of saturated fat. Similar interactions, though not significant, were shown with muscle mass, whereby individuals who were homozygous for the risk allele had lower muscle mass with higher intakes of saturated fat, a result that, to our knowledge, has not been previously reported.
与大量关于肥胖的基因研究相比，饮食与肥胖中单核苷酸多态性（SNP）之间相互作用的营养遗传学研究要少得多，尤其是在东地中海人群中。本研究的目的是评估三个候选SNP，即[具体基因名称1]基因中的[具体SNP名称1]和[具体基因名称2]基因的[具体SNP名称2]变体与肥胖、体脂和肌肉组成方面的常量营养素摄入之间的潜在相互作用。本研究纳入了308名健康的黎巴嫩成年人。数据收集包括一份关于人口统计学和生活方式的问卷，以及使用一份针对特定文化的80项半定量食物频率问卷进行的详细饮食评估。这与人体测量以及采集外周血用于DNA提取和使用Taqman等位基因鉴别分析进行基因分型相结合。在这个人群样本中，这两个[具体基因名称]候选SNP与肥胖风险无关，但在任一[具体SNP名称]的风险等位基因携带者中，存在肌肉量较低的趋势，尽管不显著。据我们所知，这些结果此前尚未见报道。至于[具体SNP名称3]变体，结果与文献一致，因为风险等位基因纯合的个体尽管饱和脂肪摄入量较低，但体重指数（BMI）和体脂显著更高。在肌肉量方面也显示出类似的相互作用，尽管不显著，即风险等位基因纯合的个体在饱和脂肪摄入量较高时肌肉量较低，据我们所知，这一结果此前尚未见报道。
