Shin Youn Ho, Kim Ki Eun, Lee Yong-Jae, Nam Jae-Hwan, Hong Young Mi, Shin Hye-Jung
Department of Pediatrics, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul, Korea. ; Department of Medicine, The Graduate School, Yonsei University, Seoul, Korea.
Department of Pediatrics, CHA Gangnam Medical Center, CHA University School of Medicine, Seoul, Korea.
Korean J Pediatr. 2014 Dec;57(12):526-32. doi: 10.3345/kjp.2014.57.12.526. Epub 2014 Dec 31.
Matrix metalloproteinases (MMPs) have been implicated in atherosclerosis, and therefore, are considered risk factors for metabolic dysfunction in adults. However, there is little data on circulating levels of MMPs and tissue inhibitors of MMPs (TIMPs) with regard to obesity-related biomarkers in the general adolescent population. In the present study, we determined the associations of MMP-8, MMP-9, and TIMP-1 levels and MMP-8/TIMP-1 and MMP-9/TIMP-1 ratios with obesity-related biomarkers in apparently healthy adolescent boys.
We measured MMP and TIMP concentrations in plasma samples using the enzyme-linked immunosorbent assay and analyzed their associations with obesity-related biomarkers, such as liver enzymes and lipid profiles, in a sample of 91 Korean boys aged 13-14 years who participated in a general health check-up.
The mean age of the boys was 13.8±0.3 years; 72 boys were normal weight and 19 were overweight/obese. The Pearson correlation coefficients revealed a significant correlation between MMP-8 and aspartate aminotransferase (r=0.217, P=0.039) and alanine aminotransferase (r=0.250, P=0.017) and between TIMP-1 and aspartate aminotransferase (r=0.267, P=0.011). In a multivariate linear regression analysis, serum alanine aminotransferase was positively associated with the MMP-8 level. There were no significant differences in the MMP-8, MMP-9, and TIMP-1 levels or MMP-8/TIMP-1 and MMP-9/TIMP-1 ratios between control and overweight/obese subjects.
We found a significant association between the MMP-8 level and alanine aminotransferase in the apparently healthy adolescent boys. These findings indicate that there may be a pathophysiological mechanism underlying the relationship between MMP-8 and liver enzymes in young adolescents.
基质金属蛋白酶（MMPs）与动脉粥样硬化有关，因此被认为是成人代谢功能障碍的危险因素。然而，关于一般青少年人群中MMPs及其组织抑制剂（TIMPs）的循环水平与肥胖相关生物标志物的数据很少。在本研究中，我们确定了明显健康的青春期男孩中MMP-8、MMP-9和TIMP-1水平以及MMP-8/TIMP-1和MMP-9/TIMP-1比值与肥胖相关生物标志物之间的关联。
我们使用酶联免疫吸附测定法测量血浆样本中的MMP和TIMP浓度，并分析了它们与肥胖相关生物标志物（如肝酶和血脂谱）之间的关联，研究对象为91名参加一般健康检查的13 - 14岁韩国男孩。
男孩的平均年龄为13.8±0.3岁；72名男孩体重正常，19名超重/肥胖。Pearson相关系数显示MMP-8与天冬氨酸转氨酶（r = 0.217，P = 0.039）和丙氨酸转氨酶（r = 0.250，P = 0.017）之间以及TIMP-1与天冬氨酸转氨酶（r = 0.267，P = 0.011）之间存在显著相关性。在多变量线性回归分析中，血清丙氨酸转氨酶与MMP-8水平呈正相关。对照组与超重/肥胖组之间的MMP-8、MMP-9和TIMP-1水平或MMP-8/TIMP-1和MMP-9/TIMP-1比值无显著差异。
我们发现明显健康的青春期男孩中MMP-8水平与丙氨酸转氨酶之间存在显著关联。这些发现表明，青少年中MMP-8与肝酶之间的关系可能存在病理生理机制。
