Physical Chemistry I, Department of Chemistry and Biology, University of Siegen , Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.
Biomacromolecules. 2015 Mar 9;16(3):832-41. doi: 10.1021/bm501729h. Epub 2015 Feb 19.
We introduce a new hyaluronidase-responsive amphiphilic block copolymer system, based on hyaluronic acid (HYA) and polycaprolactone (PCL), that can be assembled into polymersomes by an inversed solvent shift method. By exploiting the triggered release of encapsulated dye molecules, these HYA-block-PCL polymersomes lend themselves as an autonomous sensing system for the detection of the presence of hyaluronidase, which is produced among others by the pathogenic bacterium Staphylococcus aureus. The synthesis of the enzyme-responsive HYA-block-PCL block copolymers was carried out by copper-catalyzed Huisgen 1,3-dipolar cycloaddition of ω-azide-terminated PCL and ω-alkyne-functionalized HYA. The structure of the HYA-block-PCL assemblies and their enzyme-triggered degradation and concomitant cargo release were investigated by dynamic light scattering, fluorescence spectroscopy, confocal laser-scanning microscopy, scanning and transmission electron, and atomic force microscopy. As shown, a wide range of reporter dye molecules as well as antimicrobials can be encapsulated into the vesicles during formation and are released upon the addition of hyaluronidase.
我们介绍了一种新的透明质酸（HYA）和聚己内酯（PCL）的基于透明质酸（HYA）和聚己内酯（PCL）的新型透明质酸酶响应两亲嵌段共聚物系统，该系统可通过反溶剂转移方法组装成聚合物囊泡。通过利用包封染料分子的触发释放，这些 HYA-嵌段-PCL 聚合物囊泡本身可用作自主感测系统，用于检测透明质酸酶的存在，透明质酸酶除其他外由致病性细菌金黄色葡萄球菌产生。酶响应的 HYA-嵌段-PCL 嵌段共聚物的合成是通过 ω-叠氮端 PC L 和 ω-炔基官能化 HYA 的铜催化 Huisgen 1,3-偶极环加成反应进行的。通过动态光散射、荧光光谱、共聚焦激光扫描显微镜、扫描和透射电子显微镜以及原子力显微镜研究了 HYA-嵌段-PCL 组装体及其酶触发的降解和伴随的货物释放。结果表明，在形成过程中可以将广泛的报告染料分子以及抗菌剂包封在囊泡中，并在加入透明质酸酶时释放。
