Lukácová M, Brezina R, Schramek S, Pastorek J
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Acta Virol. 1989 Jan;33(1):75-80.
Optimal conditions of extraction (time and temperature) by trichloroacetic acid of soluble antigen from phase I Coxiella burnetii (TCAE), possessing protective properties and used as a chemovaccine against Q fever in men, were studied. Extracts prepared under various conditions were analysed for their polysaccharide, protein and phosphorus contents. Forty-five min of extraction at 0 degrees C were sufficient to obtain a soluble antigen reacting in immunodiffusion with hyperimmune rabbit antiserum. The polysaccharide contents decreased with prolonged extraction at 0 degrees C. At higher extraction temperatures (37 and 100 degrees C), the polysaccharide contents increased while that of proteins decreased. TCAE prepared at 100 degrees C gave no positive immunodiffusion reaction.
研究了从Ⅰ相伯纳特立克次氏体中提取具有保护特性且用作人Q热化学疫苗的可溶性抗原的三氯乙酸提取最佳条件（时间和温度）。分析了在不同条件下制备的提取物的多糖、蛋白质和磷含量。在0℃提取45分钟足以获得在免疫扩散中能与超免疫兔抗血清发生反应的可溶性抗原。在0℃下延长提取时间，多糖含量会降低。在较高提取温度（37℃和100℃）下，多糖含量增加而蛋白质含量降低。在100℃制备的三氯乙酸提取物未产生阳性免疫扩散反应。
