Lukácová M, Schramek S
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Acta Virol. 1988 Sep;32(5):461-3.
Kinetics of phospholipid extraction from purified suspensions of Coxiella burnetii in phase I by various chloroform--methanol mixtures at various temperatures were evaluated based on the amount of phosphorus extracted. Extraction by a boiling (53 degrees C) 2:1 chloroform:methanol mixture proved to be the most efficient.
基于磷的提取量,评估了在不同温度下,各种氯仿 - 甲醇混合物从处于第一阶段的纯化伯氏考克斯氏体悬浮液中提取磷脂的动力学。结果表明,用沸腾(53摄氏度)的2:1氯仿:甲醇混合物进行提取效率最高。