Han Hui, Qu Guangjin, Han Chenghua, Wang Yuhong, Sun Tingting, Li Fengqing, Wang Junxiao, Luo Shanshun
Department of Gerontology, the First Hospital of Harbin Medical University, Harbin, China.
Department of Natural Product Chemistry, the Daqing Campus of Harbin Medical University, Daqing, China.
Exp Mol Med. 2015 Feb 6;47(2):e138. doi: 10.1038/emm.2014.81.
The aim of this study was to investigate the expression of circulating microRNAs (miRNAs) in apolipoprotein E (apoE) knockout mice (apoE(-/-)) and to validate the role of these miRNAs in human coronary artery disease (CAD). Pooled plasma from 10 apoE(-/-) mice and 10 healthy C57BL/6 (B6) mice was used to perform the microarray analysis. The results showed that miR-34a, miR-21, miR-23a, miR-30a and miR-106b were differentially expressed in apoE(-/-) mice, and these expression changes were confirmed by real-time quantitative reverse-transcription PCR. Then, miR-34a, miR-21, miR-23a, miR-30a and miR-106b were detected in the plasma of 32 patients with CAD and of 20 healthy controls. Only miR-34a, miR-21 and miR-23a were significantly differentially expressed in the plasma of CAD patients (all P<0.01). In conclusion, miR-34a, miR-21 and miR-23a were elevated in CAD patients, which means that these miRNAs might serve as biomarkers of CAD development and progression.
本研究的目的是调查载脂蛋白E（apoE）基因敲除小鼠（apoE(-/-)）中循环微RNA（miRNA）的表达情况，并验证这些miRNA在人类冠状动脉疾病（CAD）中的作用。使用来自10只apoE(-/-)小鼠和10只健康C57BL/6（B6）小鼠的混合血浆进行微阵列分析。结果显示，miR-34a、miR-21、miR-23a、miR-30a和miR-106b在apoE(-/-)小鼠中差异表达，并且这些表达变化通过实时定量逆转录PCR得到证实。然后，在32例CAD患者和20例健康对照者的血浆中检测了miR-34a、miR-21、miR-23a、miR-30a和miR-106b。只有miR-34a、miR-21和miR-23a在CAD患者血浆中显著差异表达（均P<0.01）。总之，CAD患者中miR-34a、miR-21和miR-23a升高，这意味着这些miRNA可能作为CAD发生和进展的生物标志物。
