Haghi G, Hatami A, Mehran M, Hosseini H
Phytochemistry Group, Barij Essence Medicinal Plants Research Center, Kashan, I.R. Iran.
Agriculture Group, Barij Essence Medicinal Plants Research Center, Kashan, I.R. Iran.
Res Pharm Sci. 2014 Sep-Oct;9(5):323-30.
In this study, caffeic acid (CA) and its three derivatives including 3-caffeoylquinic acid (3-CQA, neochlorogenic acid), 4-caffeoylquinic acid (4-CQA, cryptochlorogenic acid), and 5-caffeoylquinic acid (5-CQA, chlorogenic acid) were identified in Bupleurum chinense aerial parts using reverse-phase high-performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detector, reference compounds and chemical reactions. Separation was performed on a C18 column using gradient elution with 4% (v/v) aqueous acetic acid and acetonitrile as mobile phase at ambient temperature. In addition, the flavonoid aglycones were characterized and quantified after acid hydrolysis of the plant material. The flavonols profile showed quercetin (0.36 g per 100 g), kaempferol (1.11 g per 100 g) and isorhamnetin (0.16 g per 100 g). Total phenolic and total flavonoid contents ranged from 7.3 to 18.7% and 0.58 to 2.72% in dry plant material, respectively.
在本研究中，采用带光电二极管阵列（PDA）检测器的反相高效液相色谱（RP-HPLC）、对照品和化学反应，在柴胡地上部分鉴定出了咖啡酸（CA）及其三种衍生物，包括3-咖啡酰奎尼酸（3-CQA，新绿原酸）、4-咖啡酰奎尼酸（4-CQA，隐绿原酸）和5-咖啡酰奎尼酸（5-CQA，绿原酸）。在室温下，使用4%（v/v）的乙酸水溶液和乙腈作为流动相，在C18柱上进行梯度洗脱分离。此外，对植物材料进行酸水解后，对黄酮苷元进行了表征和定量。黄酮醇谱显示含有槲皮素（每100克0.36克）、山奈酚（每100克1.11克）和异鼠李素（每100克0.16克）。干燥植物材料中总酚和总黄酮含量分别为7.3%至18.7%和0.58%至2.72%。
