Mojarrab M, Mohammadi A Khan, Hosseinzadeh L, Siavash-Haghighi Z Minoosh
Novel Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
Students research committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.
Res Pharm Sci. 2014 Sep-Oct;9(5):359-66.
Tragopogon buphthalmoides (DC.) Boiss, is widely used as a food additive with some imputed health effects in folk medicine of western Iran. Unfortunately, despite the prevalent medicinal uses of the plant, there are no reports on the toxic effects of T. buphthalmoides aerial parts. The present study evaluated the potential toxicity of dried hydroethanolic extract of the species in wistar rats. Also, we investigated antioxidant activity and total phenolic content (TPC) of the extract. In the acute study, single doses of extract were administered orally, and the rats were then monitored for 14 days. In the subchronic toxicity study, the sample was administered to the rats for 45 days. In the antioxidant capacity assays dried extract showed moderate to weak antioxidant activities. Also, the sample showed relatively notable TPC. The results of acute study indicated that the LD50 of T. buphthalmoides is higher than 2500 mg/kg. Biochemical analysis showed some significant changes including creatinine, glucose and triglyceride levels. Moreover, some significant abnormality of lung, kidney and liver organs was observed. Based on the results of this study, adverse effect level (AEL) of dried hydroethanolic extract of T. buphthalmoides considered to be less than 175 mg/kg/day for the male and female rats.
伊朗西部民间医学中，假婆罗门参（Tragopogon buphthalmoides (DC.) Boiss）被广泛用作食品添加剂，据说具有一定的健康功效。遗憾的是，尽管该植物有广泛的药用价值，但关于其地上部分毒性作用的报道却未见踪影。本研究评估了该物种干燥水乙醇提取物对Wistar大鼠的潜在毒性。此外，我们还研究了提取物的抗氧化活性和总酚含量（TPC）。在急性研究中，口服单剂量提取物，然后对大鼠进行14天监测。在亚慢性毒性研究中，将样品给予大鼠45天。在抗氧化能力测定中，干燥提取物表现出中度至弱的抗氧化活性。此外，样品显示出相对显著的TPC。急性研究结果表明，假婆罗门参的半数致死剂量（LD50）高于2500 mg/kg。生化分析显示了一些显著变化，包括肌酐、葡萄糖和甘油三酯水平。此外，还观察到肺、肾和肝器官出现一些显著异常。基于本研究结果，假婆罗门参干燥水乙醇提取物对雄性和雌性大鼠的不良反应水平（AEL）被认为低于175 mg/kg/天。
