通过光声检测的非接触式声辐射力脉冲显微镜用于探测乳腺癌细胞力学。
Non-contact acoustic radiation force impulse microscopy via photoacoustic detection for probing breast cancer cell mechanics.
作者信息
Hwang Jae Youn, Kang Bong Jin, Lee Changyang, Kim Hyung Ham, Park Jinhyoung, Zhou Qifa, Shung K Kirk
机构信息
Department of Information and Communication Engineering, Daegu Gyeongbuk Institute of Science & Technology, Daegu, South Korea.
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
出版信息
Biomed Opt Express. 2014 Dec 3;6(1):11-22. doi: 10.1364/BOE.6.000011. eCollection 2015 Jan 1.
We demonstrate a novel non-contact method: acoustic radiation force impulse microscopy via photoacoustic detection (PA-ARFI), capable of probing cell mechanics. A 30 MHz lithium niobate ultrasound transducer is utilized for both detection of phatoacoustic signals and generation of acoustic radiation force. To track cell membrane displacements by acoustic radiation force, functionalized single-walled carbon nanotubes are attached to cell membrane. Using the developed microscopy evaluated with agar phantoms, the mechanics of highly- and weakly-metastatic breast cancer cells are quantified. These results clearly show that the PA-ARFI microscopy may serve as a novel tool to probe mechanics of single breast cancer cells.
我们展示了一种新型非接触方法
通过光声检测的声辐射力脉冲显微镜（PA-ARFI），它能够探测细胞力学。一个30兆赫的铌酸锂超声换能器用于光声信号检测和声辐射力的产生。为了通过声辐射力追踪细胞膜位移，将功能化的单壁碳纳米管附着到细胞膜上。使用经琼脂体模评估的已开发显微镜，对高转移性和低转移性乳腺癌细胞的力学特性进行了量化。这些结果清楚地表明，PA-ARFI显微镜可作为探测单个乳腺癌细胞力学特性的新型工具。
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