Kuo Jinn-Rung, Lin Shih-Shian, Liu Janelle, Chen Shih-How, Chio Chung-Chin, Wang Jhi-Joung, Liu Jia-Ming
Departments of Neurosurgery, Chi-Mei Medical Center, Tainan, Taiwan ; Department of Biotechnology, Southern Taiwan University of Science and Technology, Tainan, Taiwan.
Neurophotonics Laboratory, College of Photonics, National Chiao Tung University, Tainan, Taiwan.
Biomed Opt Express. 2014 Dec 3;6(1):23-31. doi: 10.1364/BOE.6.000023. eCollection 2015 Jan 1.
Compared to deep brain electrical stimulation, which has been applied to treating pathological brain diseases, little work has been done on the effect of deep brain light stimulation. A fiber-coupled laser stimulator at 840 nm wavelength and 130 Hz pulse repetition rate is developed in this work for deep brain light stimulation in a rat model. Concentration changes in glutamate and dopamine in the striatum are observed using a microdialysis probe when the subthalamic nucleus (STN) is stimulated at various optical power levels. Experimental results show that light stimulation causes the concentration of glutamate to decrease while that of dopamine is increased. This suggests that deep brain light stimulation of the STN is a promising therapeutic strategy for dopamine-related diseases such as Parkinson's disease. The stimulator developed for this work is useful for deep brain light stimulation in biomedical research.
与已应用于治疗脑部疾病的深部脑电刺激相比，关于深部脑光刺激的效果研究较少。在这项工作中，开发了一种波长为840 nm、脉冲重复频率为130 Hz的光纤耦合激光刺激器，用于大鼠模型中的深部脑光刺激。当在不同光功率水平刺激丘脑底核（STN）时，使用微透析探针观察纹状体中谷氨酸和多巴胺的浓度变化。实验结果表明，光刺激会使谷氨酸浓度降低，而多巴胺浓度升高。这表明对STN进行深部脑光刺激是治疗帕金森病等多巴胺相关疾病的一种有前景的治疗策略。为这项工作开发的刺激器可用于生物医学研究中的深部脑光刺激。
