Chaubaroux Christophe, Perrin-Schmitt Fabienne, Senger Bernard, Vidal Loïc, Voegel Jean-Claude, Schaaf Pierre, Haikel Youssef, Boulmedais Fouzia, Lavalle Philippe, Hemmerlé Joseph
1 Institut National de la Santé et de la Recherche Médicale , UMR-S 1121, "Biomaterials and Bioengineering", Strasbourg, France .
2 Faculté de Chirurgie Dentaire, Université de Strasbourg , Strasbourg, France .
Tissue Eng Part C Methods. 2015 Sep;21(9):881-8. doi: 10.1089/ten.TEC.2014.0479. Epub 2015 Mar 17.
For many years it has been a major challenge to regenerate damaged tissues using synthetic or natural materials. To favor the healing processes after tendon, cornea, muscle, or brain injuries, aligned collagen-based architectures are of utmost interest. In this study, we define a novel aligned coating based on a collagen/alginate (COL/ALG) multilayer film. The coating exhibiting a nanofibrillar structure is cross-linked with genipin for stability in physiological conditions. By stretching COL/ALG-coated polydimethylsiloxane substrates, we developed a versatile method to align the collagen fibrils of the polymeric coating. Assays on cell morphology and alignment were performed to investigate the properties of these films. Microscopic assessments revealed that cells align with the stretched collagen fibrils of the coating. The degree of alignment is tuned by the stretching rate (i.e., the strain) of the COL/ALG-coated elastic substrate. Such coatings are of great interest for strategies that require aligned nanofibrillar biological material as a substrate for tissue engineering.
多年来，使用合成材料或天然材料再生受损组织一直是一项重大挑战。为了促进肌腱、角膜、肌肉或脑部损伤后的愈合过程，基于胶原蛋白的排列结构备受关注。在本研究中，我们定义了一种基于胶原蛋白/藻酸盐（COL/ALG）多层膜的新型排列涂层。具有纳米纤维结构的涂层与京尼平交联，以在生理条件下保持稳定性。通过拉伸COL/ALG涂层的聚二甲基硅氧烷基材，我们开发了一种通用方法来排列聚合物涂层中的胶原纤维。进行了细胞形态和排列分析以研究这些薄膜的性能。显微镜评估显示，细胞与涂层中拉伸的胶原纤维对齐。对齐程度由COL/ALG涂层弹性基材的拉伸速率（即应变）调节。这种涂层对于需要排列的纳米纤维生物材料作为组织工程基材的策略非常有意义。
