Institut National de la Santé et de la Recherche Médicale , INSERM UMR 977, Biomaterials and Tissue Engineering, Strasbourg, France.
Biomacromolecules. 2012 Jul 9;13(7):2128-35. doi: 10.1021/bm300529a. Epub 2012 Jun 13.
Surface functionalization plays an important role in the design of biomedical implants, especially when layer forming cells, such as endothelial or epithelial cells, are needed. In this study, we define a novel nanoscale surface coating composed of collagen/alginate polyelectrolyte multilayers and cross-linked for stability with genipin. This buildup follows an exponential growth regime versus the number of deposition cycles with a distinct nanofibrillar structure that is not damaged by the cross-linking step. Stability and cell compatibility of the cross-linked coatings were studied with human umbilical vein endothelial cells. The surface coating can be covered by a monolayer of vascular endothelial cells within 5 days. Genipin cross-linking renders the surface more suitable for cell attachment and proliferation compared to glutaraldehyde (more conventional cross-linker) cross-linked surfaces, where cell clumps in dispersed areas were observed. In summary, it is possible with the defined system to build fibrillar structures with a nanoscale control of film thickness, which would be useful for in vivo applications such as inner lining of lumens for vascular and tracheal implants.
表面功能化在生物医学植入物的设计中起着重要作用，特别是在需要形成细胞层，如内皮细胞或上皮细胞时。在这项研究中，我们定义了一种由胶原/海藻酸钠聚电解质多层组成的新型纳米级表面涂层，并通过京尼平交联以增加其稳定性。这种构建方式遵循与沉积循环次数呈指数增长的关系，具有独特的纳米纤维结构，交联步骤不会破坏该结构。我们用人脐静脉内皮细胞研究了交联涂层的稳定性和细胞相容性。交联涂层可在 5 天内被单层血管内皮细胞覆盖。与戊二醛（更传统的交联剂）交联表面相比，京尼平交联使表面更适合细胞附着和增殖，因为在戊二醛交联表面上观察到分散区域中的细胞团块。总之，通过定义的系统，可以构建具有纳米级薄膜厚度控制的纤维状结构，这对于血管和气管植入物的管腔内部衬里等体内应用非常有用。
