Renalier M H, Joseph N, Bachellerie J P
Centre de Recherche en Biochimie et Génétique Cellulaires du CNRS, Université Paul-Sabatier, Toulouse, France.
FEBS Lett. 1989 Apr 24;247(2):298-302. doi: 10.1016/0014-5793(89)81356-0.
In order to assess the extent of structural heterogeneity among ribosomal genes in the human genome, several cosmid clones, each containing an entire transcription unit, have been isolated and analyzed. Five cloned genes were recognized as structurally different from each other to some extent, either within the transcription unit or in its immediate vicinity. The sequence of a 1.2 kb region encompassing the transcription start site has been determined for the five cloned genes. Point differences among the genes are observed at five nucleotide positions within the analyzed portion of the 5' external transcribed spacer of the ribosomal gene. Moreover, upstream from the transcription start site, a unique difference is observed with the absence from the three genes of a 19-nucleotide long stretch which is present in the two other variant gene forms.
为了评估人类基因组中核糖体基因之间结构异质性的程度，已分离并分析了几个黏粒克隆，每个克隆都包含一个完整的转录单元。五个克隆基因在转录单元内部或其紧邻区域在一定程度上被认为彼此结构不同。已确定了五个克隆基因中包含转录起始位点的1.2 kb区域的序列。在核糖体基因5'外部转录间隔区分析部分的五个核苷酸位置观察到基因之间的点差异。此外，在转录起始位点上游，观察到一个独特的差异，即三个基因中不存在另外两种变异基因形式中存在的一段19个核苷酸长的序列。
