小鼠核糖体RNA基因重复序列的组织

Kominami R, Urano Y, Mishima Y, Muramatsu M

Nucleic Acids Res. 1981 Jul 24;9(14):3219-33. doi: 10.1093/nar/9.14.3219.

The organization of the ribosomal RNA (rRNA) genes of the mouse was determined by Southern blot hybridization using cloned rDNA fragments as probes, which could encompass the entire spacer region between two rRNA gene regions. The rRNA genes are organized into tandem repeats of nearly uniform length of about 44 kb. The heterogeneity detected in the nontranscribed spacer appears to be caused by its sequence rather than its length difference. At least three kinds of repetitive sequences are present in the non-transcribed spacer region; two of them are located 13 kb upstream from the 5'-end of 18S RNA gene and the other located 1 to 4 kb downstream from the 3'-end of 28S RNA gene.

利用克隆的rDNA片段作为探针，通过Southern印迹杂交确定了小鼠核糖体RNA（rRNA）基因的组织方式，这些探针可以覆盖两个rRNA基因区域之间的整个间隔区。rRNA基因被组织成长度近44 kb的串联重复序列。在非转录间隔区检测到的异质性似乎是由其序列而非长度差异引起的。在非转录间隔区至少存在三种重复序列；其中两种位于18S RNA基因5'端上游13 kb处，另一种位于28S RNA基因3'端下游1至4 kb处。