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非洲爪蟾和北方爪蟾核糖体基因间隔区的序列组织

Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealis.

作者信息

Bach R, Allet B, Crippa M

出版信息

Nucleic Acids Res. 1981 Oct 24;9(20):5311-30. doi: 10.1093/nar/9.20.5311.

DOI:10.1093/nar/9.20.5311
PMID:6272218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327522/
Abstract

We have studied in X. clivii and X. borealis cloned EcoRI fragments containing the spacer located between the 28S and 18S ribosomal genes. We report for these two species the nucleotide sequences at both ends of the NTS region with special emphasis on the sequences around the transcription initiation site of the 40S rRNA precursor. In X. clivii the location of the 5' end of the precursor was mapped. In both species the sequences around the 40S origin are duplicated in the NTS. Nucleotide sequence comparison has revealed a stretch of 13 identical bases around the transcription initiation site of X. laevis and X. clivii. The same sequence is also present at the presumptive transcription initiation site of X. borealis rDNA.

摘要

我们研究了光滑爪蟾（X. clivii）和北方爪蟾（X. borealis）中包含位于28S和18S核糖体基因之间间隔区的克隆EcoRI片段。我们报告了这两个物种NTS区域两端的核苷酸序列，特别强调了40S rRNA前体转录起始位点周围的序列。在光滑爪蟾中，前体5'端的位置已被定位。在这两个物种中，40S起始位点周围的序列在NTS中是重复的。核苷酸序列比较显示，在非洲爪蟾（X. laevis）和光滑爪蟾转录起始位点周围有一段13个相同碱基的序列。相同的序列也存在于北方爪蟾rDNA的假定转录起始位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3111/327522/3a00a42b183b/nar00413-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3111/327522/3a00a42b183b/nar00413-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3111/327522/3a00a42b183b/nar00413-0140-a.jpg

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