一种通过组氨酸选择性金属化构建的人工一氧化碳释放金属蛋白。
An artificial CO-releasing metalloprotein built by histidine-selective metallation.
作者信息
Albuquerque Inês S, Jeremias Hélia F, Chaves-Ferreira Miguel, Matak-Vinkovic Dijana, Boutureira Omar, Romão Carlos C, Bernardes Gonçalo J L
机构信息
Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal.
出版信息
Chem Commun (Camb). 2015 Mar 7;51(19):3993-6. doi: 10.1039/c4cc10204e.
We report the design and synthesis of an aquacarbonyl Ru(II) dication cis-Ru(CO)2(H2O)4 reagent for histidine (His)-selective metallation of interleukin (IL)-8 at site 33. The artificial, non-toxic interleukin (IL)-8-Ru(II)(CO)2 metalloprotein retained IL-8-dependent neutrophil chemotactic activity and was shown to spontaneously release CO in live cells.
我们报道了一种用于对白细胞介素（IL）-8的33位组氨酸（His）进行选择性金属化的水合羰基钌（II）二价阳离子顺式-Ru(CO)₂(H₂O)₄试剂的设计与合成。人工合成的无毒白细胞介素（IL）-8-Ru(II)(CO)₂金属蛋白保留了IL-8依赖性中性粒细胞趋化活性，并被证明能在活细胞中自发释放一氧化碳。
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