Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Food Chem. 2015 Jun 15;177:111-9. doi: 10.1016/j.foodchem.2015.01.017. Epub 2015 Jan 8.
Two real-time polymerase chain reaction (PCR)-based assays for detection of walnut (Juglans regia) and pecan (Carya illinoinensis) traces in a wide range of processed foods are described here. The method consists on a real-time PCR assay targeting the ITS1 region, using a nuclease (TaqMan) probe labeled with FAM and BBQ. The method was positive for walnut and pecan respectively, and negative for all other heterologous plants and animals tested. Using a series of model samples with defined raw walnut in wheat flour and heat-treated walnut in wheat flour with a range of concentrations of 0.1-100,000 mg kg(-1), a practical detection limit of 0.1 mg kg(-1) of walnut content was estimated. Identical binary mixtures were done for pecan, reaching the same limit of detection of 0.1 mg kg(-1). The assay was successfully trialed on a total of 232 commercial foodstuffs.
本文介绍了两种用于检测多种加工食品中胡桃（Juglans regia）和山核桃（Carya illinoinensis）痕迹的实时聚合酶链反应（PCR）检测方法。该方法基于针对 ITS1 区域的实时 PCR 检测，使用带有 FAM 和 BBQ 标记的核酸酶（TaqMan）探针。该方法分别对胡桃和山核桃呈阳性，而对所有其他异源植物和动物均呈阴性。使用一系列含有定义量的生胡桃的小麦粉模型样品和经过热处理的胡桃的小麦粉模型样品，其浓度范围为 0.1-100,000 mg kg(-1)，估计胡桃含量的实际检测限为 0.1 mg kg(-1)。对山核桃进行了相同的二元混合物分析，达到了相同的 0.1 mg kg(-1)检测限。该检测方法总共在 232 种商业食品上进行了试用。
