Council of Scientific and Industrial Research-Institute of Himalayan Bioresource Technology, Palampur, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Phytochem Anal. 2024 Jan;35(1):102-115. doi: 10.1002/pca.3278. Epub 2023 Sep 11.
Juglans regia Linn. and Carya illinoinensis (Wangenh.) K. Koch are nut-producing plant species of the Juglandaceae family. Bioactive compounds like naphthoquinones, tetralones, and diarylheptanoids are dominant in these species.
The study aimed to develop and validate a fast and sensitive analytical method by ultrahigh-performance liquid chromatography diode array detection mass spectrometry (UHPLC-DAD-MS) for quantification and identification of bioactive compounds in fruit pericarps and leaves of J. regia and C. illinoinensis collected from two different states of north India.
The dried pericarps of J. regia and C. illinoinensis (500 mg) were extracted with ethyl acetate-methanol (50:50 v/v, 20 mL, 50°C, 30 min) by ultrasonication and analysed by ultrahigh-performance liquid chromatography diode array detection quadrupole time-of-flight tandem mass spectrometry (UHPLC-DAD-QTOF-MS/MS) for qualitative and quantitative examination of phytoconstituents. The method was validated according to International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human use (ICH) guidelines for linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ).
Here, we report the quantification of dihydrophaseic acid (1), 4,5-dihydroxytetralone (2), 4,8-dihydroxytetralone (3), 5,8-dihydroxy-3-methoxytetralone (4), and juglanin A (5) in the pericarps and leaves of J. regia and C. illinoinensis. Furthermore, using the hyphenated analytical method, a total of 62 compounds were tentatively characterised in different samples. The principal component analysis (PCA) showed diversity between the analysed sample's composition. Also, the study evaluated the variation of bioactive compounds among different parts of J. regia and C. illinoinensis collected from different regions of northern India by UHPLC-DAD-QTOF-MS/MS.
The developed method is simple, rapid, and selective for the identification and characterisation of bioactive molecules.
胡桃（Juglans regia Linn.）和薄壳山核桃（Carya illinoinensis (Wangenh.) K. Koch）是胡桃科的产核植物种。这些物种中含有萘醌、四氢萘酮和二芳基庚烷类等生物活性化合物。
本研究旨在建立并验证一种快速灵敏的分析方法，通过超高效液相色谱二极管阵列检测质谱法（UHPLC-DAD-MS）对来自印度北部两个不同地区的胡桃和薄壳山核桃果实种皮和叶片中的生物活性化合物进行定量和鉴定。
将胡桃和薄壳山核桃的干燥种皮（500mg）用乙酸乙酯-甲醇（50:50v/v，20mL，50°C，30min）超声提取，并用超高效液相色谱二极管阵列检测四级杆飞行时间串联质谱法（UHPLC-DAD-QTOF-MS/MS）进行分析，以定性和定量检查植物成分。该方法按照国际人用药品注册技术协调会（ICH）指南进行了线性、精密度、准确度、检测限（LOD）和定量限（LOQ）的验证。
本研究报告了在胡桃和薄壳山核桃的种皮和叶片中，二氢表儿茶酸（1）、4,5-二羟基四氢萘酮（2）、4,8-二羟基四氢萘酮（3）、5,8-二羟基-3-甲氧基四氢萘酮（4）和胡桃苷 A（5）的定量分析。此外，通过联用分析方法，共在不同样品中初步鉴定了 62 种化合物。主成分分析（PCA）表明了分析样品组成之间的多样性。此外，该研究还通过 UHPLC-DAD-QTOF-MS/MS 评估了来自印度北部不同地区的胡桃和薄壳山核桃不同部位生物活性化合物的变化。
所建立的方法简单、快速、选择性强，可用于生物活性分子的鉴定和表征。
