Nanotechnology and Catalysis Research Centre (NanoCat), University of Malaya, Kuala Lumpur 50603, Malaysia; Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur 50603, Malaysia.
Nanotechnology and Catalysis Research Centre (NanoCat), University of Malaya, Kuala Lumpur 50603, Malaysia.
Food Chem. 2015 Jun 15;177:214-24. doi: 10.1016/j.foodchem.2014.12.098. Epub 2015 Jan 2.
Food falsification has direct impact on public health, religious faith, fair-trades and wildlife. For the first time, here we described a multiplex polymerase chain reaction assay for the accurate identification of five meat species forbidden in Islamic foods in a single assay platform. Five pairs of species-specific primers were designed targeting mitochondrial ND5, ATPase 6, and cytochrome b genes to amplify 172, 163, 141, 129 and 108 bp DNA fragments from cat, dog, pig, monkey and rat meats, respectively. All PCR products were identified in gel-images and electrochromatograms obtained from Experion Bioanalyzer. Species-specificity checking against 15 important meat and fish and 5 plant species detected no cross-species amplification. Screening of target species in model and commercial meatballs reflected its application to detect target species in process foods. The assay was tested to detect 0.01-0.02 ng DNA under raw states and 1% suspected meats in meatball formulation.
食品伪造直接影响公众健康、宗教信仰、公平贸易和野生动物。我们首次描述了一种多重聚合酶链反应（PCR）检测方法，可在单个检测平台上准确鉴定伊斯兰教食品中禁止的五种肉类。设计了五对种特异性引物，针对线粒体 ND5、ATPase 6 和细胞色素 b 基因，分别从猫、狗、猪、猴子和老鼠肉中扩增 172、163、141、129 和 108 bp 的 DNA 片段。所有 PCR 产物均在凝胶图像和 Experion Bioanalyzer 获得的电泳图谱中得到鉴定。对 15 种重要的肉类和鱼类以及 5 种植物物种进行种特异性检查，未发现交叉物种扩增。在模型和商业肉丸中对目标物种的筛选反映了其在加工食品中检测目标物种的应用。该检测方法在原始状态下可检测到 0.01-0.02ng DNA，在肉丸配方中可检测到 1%的可疑肉类。
