Yang Seung-Min, Kim Jun-Su, Kim Eiseul, Kim Hae-Yeong
Institute of Life Sciences & Resources, Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Foods. 2024 Jan 23;13(3):363. doi: 10.3390/foods13030363.
Lavers are typically consumed in dried or seasoned forms. However, commercially processed lavers can lead to seafood fraud because it is impossible to authenticate the original species based on morphological characteristics alone. In this study, we developed a capillary electrophoresis-based multiplex polymerase chain reaction (PCR) to authenticate six different laver species. The species-specific primer sets to target the chloroplast or genes were newly designed. We successfully established both singleplex and multiplex conditions, which resulted in specific amplicons for each species (, 274 bp; , 211 bp; , 195 bp; , 169 bp; , 127 bp; , 117 bp). Moreover, the assays were sensitive enough to detect DNA ranging from 10 to 0.1 pg of DNA. The optimized capillary electrophoresis-based multiplex PCR was successfully applied to 40 commercial laver products. In addition to detecting the laver species as stated on the commercial label, the assay discovered cases where less expensive species were mixed in. With its advantageous properties, such as short amplicon size, high specificity, and superior sensitivity, this assay could be used for the authentication of the six laver species.
紫菜通常以干燥或调味的形式食用。然而，商业加工的紫菜可能会导致海鲜欺诈，因为仅根据形态特征无法鉴定原始物种。在本研究中，我们开发了一种基于毛细管电泳的多重聚合酶链反应（PCR）来鉴定六种不同的紫菜物种。新设计了针对叶绿体或基因的物种特异性引物组。我们成功建立了单重和多重条件，每个物种都产生了特异性扩增子（，274 bp；，211 bp；，195 bp；，169 bp；，127 bp；，117 bp）。此外，这些检测方法足够灵敏，能够检测出10至0.1 pg的DNA。优化后的基于毛细管电泳的多重PCR成功应用于40种商业紫菜产品。除了检测商业标签上标明的紫菜物种外，该检测方法还发现了混入较便宜物种的情况。由于其具有扩增子尺寸短、特异性高和灵敏度高等优点，该检测方法可用于六种紫菜物种的鉴定。
