R&D Center, Ahram Biosystems Inc., Seoul 133-120, Republic of Korea; Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
R&D Center, Ahram Biosystems Inc., Seoul 133-120, Republic of Korea.
Food Chem. 2017 Aug 15;229:341-346. doi: 10.1016/j.foodchem.2017.02.085. Epub 2017 Feb 21.
The aim of this study was to develop an ultra-fast molecular detection method for meat identification using convection Palm polymerase chain reaction (PCR). The mitochondrial cytochrome b (Cyt b) gene was used as a target gene. Amplicon size was designed to be different for beef, lamb, and pork. When these primer sets were used, each species-specific set specifically detected the target meat species in singleplex and multiplex modes in a 24min PCR run. The detection limit was 1pg of DNA for each meat species. The convection PCR method could detect as low as 1% of meat adulteration. The stability of the assay was confirmed using thermal processed meats. We also showed that direct PCR can be successfully performed with mixed meats and food samples. These results suggest that the developed assay may be useful in the authentication of meats and meat products in laboratory and rapid on-site applications.
本研究旨在开发一种使用对流式掌上聚合酶链反应（PCR）的超快速分子肉类鉴定检测方法。线粒体细胞色素 b（Cyt b）基因被用作靶基因。扩增子大小设计为牛肉、羊肉和猪肉的大小不同。当使用这些引物组时，每个种特异性组在 24 分钟的 PCR 运行中以单重和多重模式特异性地检测目标肉类物种。每种肉类的检测限为 1pg DNA。对流 PCR 方法可检测到低至 1%的肉类掺假。通过热处理肉类来确认该检测方法的稳定性。我们还表明，直接 PCR 可以成功地与混合肉类和食品样品一起进行。这些结果表明，所开发的检测方法可能有助于在实验室和快速现场应用中对肉类和肉类产品进行认证。
