Fu Xianhua, Zhu Min, Sun Xiaoyang, Fang Dazhao, Wang Weijie, Dong Nan, Pang Cong, Liu Xiaoning, Chen Fengli, Ding Lianshu
Department of Neurosurgery, Huai'an First People's Hospital, Nanjing Medical University 6 Beijing Road West, Huai'an 223300, Jiangsu, China ; Department of Neurosurgery, The First People's Hospital of Suqian 260 Taihu Road, Suqian 223800, Jiangsu, China.
Department of Neurosurgery, Siyang People's Hospital 26 Zhongxing Road, Siyang 223700, Jiangsu, China.
Int J Clin Exp Med. 2014 Dec 15;7(12):5686-90. eCollection 2014.
The abnormal metabolic processes following traumatic brain injury (TBI) have been proposed to contribute to secondary injuries after TBI. Therefore, enteral nutrition (EN) support for TBI patients has received more attention. This study aimed to evaluate the complimentary effects of enteral nutrition with glutamine and hyperbaric oxygen (HBO) on the recovery of TBI. TBI model was established in SD rats, which were randomly divided into four groups: TBI, TBI + HBO, TBI + GLN, and TBI + HBO + GLN. Neuronal apoptosis in penumbra area was detected by TUNEL. Serum prealbumin level was detected by ELISA. Motor function was evaluated by beam-balance test. We found that the body weight of the rats had no significant differences in different groups before and after injury. Among the four groups, beam-balance test score was the lowest, serum prealbumin level was the highest, and neuronal apoptosis rate was the lowest in TBI + HBO + GLN group on day 3 and 7 after TBI. In conclusion, our data suggest that hyperbaric oxygen combined with enteral nutrition support with glutamine is effective in reducing neuronal apoptosis, increasing serum prealbumin concentration and improving neurological function after TBI injury.
创伤性脑损伤（TBI）后的异常代谢过程被认为会导致TBI后的继发性损伤。因此，TBI患者的肠内营养（EN）支持受到了更多关注。本研究旨在评估肠内营养联合谷氨酰胺和高压氧（HBO）对TBI恢复的补充作用。在SD大鼠中建立TBI模型，将其随机分为四组：TBI组、TBI + HBO组、TBI + GLN组和TBI + HBO + GLN组。采用TUNEL法检测半暗带区域神经元凋亡。采用ELISA法检测血清前白蛋白水平。通过平衡木试验评估运动功能。我们发现，损伤前后不同组大鼠体重无显著差异。在四组中，TBI + HBO + GLN组在TBI后第3天和第7天的平衡木试验评分最低，血清前白蛋白水平最高，神经元凋亡率最低。总之，我们的数据表明，高压氧联合谷氨酰胺肠内营养支持可有效减少TBI损伤后神经元凋亡，提高血清前白蛋白浓度并改善神经功能。
