Hadzi San, Garcia-Pino Abel, Gerdes Kenn, Lah Jurij, Loris Remy
Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):226-33. doi: 10.1107/S2053230X15000746. Epub 2015 Jan 28.
The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from Vibrio cholerae were crystallized in complex with their operator box. Screening of 22 different DNA duplexes led to two crystal forms of HigA2 complexes and one crystal form of a HigBA2 complex. Crystals of HigA2 in complex with a 17 bp DNA duplex belong to space group P3221, with unit-cell parameters a = b = 94.0, c = 123.7 Å, and diffract to 2.3 Å resolution. The second form corresponding to HigA2 in complex with a 19 bp duplex belong to space group P43212 and only diffract to 3.45 Å resolution. Crystals of the HigBA2 toxin-antitoxin were obtained in complex with a 31 bp duplex and belonged to space group P41212, with unit-cell parameters a = b = 113.6, c = 121.1 Å. They diffract to 3.3 Å resolution.
霍乱弧菌的HigA2抗毒素和HigBA2毒素-抗毒素复合物与其操纵子框形成复合物并结晶。对22种不同的DNA双链体进行筛选，得到了两种HigA2复合物晶体形式和一种HigBA2复合物晶体形式。与17 bp DNA双链体形成复合物的HigA2晶体属于空间群P3221，晶胞参数a = b = 94.0，c = 123.7 Å，衍射分辨率为2.3 Å。与19 bp双链体形成复合物的第二种HigA2晶体形式属于空间群P43212，衍射分辨率仅为3.45 Å。HigBA2毒素-抗毒素复合物晶体是与31 bp双链体形成复合物后得到的，属于空间群P41212，晶胞参数a = b = 113.6，c = 121.1 Å。它们的衍射分辨率为3.3 Å。
