Hadži San, Garcia-Pino Abel, Martinez-Rodriguez Sergio, Verschueren Koen, Christensen-Dalsgaard Mikkel, Gerdes Kenn, Lah Jurij, Loris Remy
Structural Biology Brussels, Department of Biotechnology, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussel, Belgium.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):1052-9. doi: 10.1107/S1744309113021490. Epub 2013 Aug 27.
The genome of Vibrio cholerae encodes two higBA toxin-antitoxin (TA) modules that are activated by amino-acid starvation. Here, the TA complex of the second module, higBA2, as well as the C-terminal domain of the corresponding HigA2 antitoxin, have been purified and crystallized. The HigBA2 complex crystallized in two crystal forms. Crystals of form I belonged to space group P2(1)2(1)2, with unit-cell parameters a = 129.0, b = 119.8, c = 33.4 Å, and diffracted to 3.0 Å resolution. The asymmetric unit is likely to contain a single complex consisting of two toxin monomers and one antitoxin dimer. The second crystal form crystallized in space group P3(2)21, with unit-cell parameters a = 134.5, c = 55.4 Å. These crystals diffracted to 2.2 Å resolution and probably contain a complex with a different stoichiometry. Crystals of the C-terminal domain of HigA2 belonged to space group C2, with unit-cell parameters a = 115.4, b = 61.2, c = 73.8 Å, β = 106.7°, and diffracted to 1.8 Å resolution.
霍乱弧菌的基因组编码两个由氨基酸饥饿激活的higBA毒素-抗毒素（TA）模块。在此，第二个模块的TA复合物higBA2以及相应抗毒素HigA2的C端结构域已被纯化并结晶。higBA2复合物以两种晶体形式结晶。I型晶体属于空间群P2(1)2(1)2，晶胞参数a = 129.0、b = 119.8、c = 33.4 Å，衍射分辨率为3.0 Å。不对称单元可能包含由两个毒素单体和一个抗毒素二聚体组成的单个复合物。第二种晶体形式结晶于空间群P3(2)21，晶胞参数a = 134.5、c = 55.4 Å。这些晶体衍射分辨率为2.2 Å，可能包含具有不同化学计量比的复合物。HigA2 C端结构域的晶体属于空间群C2，晶胞参数a = 115.4、b = 61.2、c = 73.8 Å，β = 106.7°，衍射分辨率为1.8 Å。
