School of Biosciences, Cardiff University, Cardiff CF10 3AX, UK; Institute of Research in Biodiversity (IRBio), Facultat de Biologia, Universitat de Barcelona, ES-08028 Barcelona, Spain.
Department of Wetland Ecology, Estación Biológica de Doñana-CSIC, ES-41092 Sevilla, Spain.
Sci Total Environ. 2015 May 1;514:322-32. doi: 10.1016/j.scitotenv.2015.02.004. Epub 2015 Feb 8.
Blood analyses have great potential in studies of ecology, ecotoxicology and veterinary science in wild vertebrates based on advances in human and domestic animal medicine. The major caveat for field researchers, however, is that the 'rules' for human or domestic animal haematology do not always apply to wildlife. The present overview shows the strengths and limitations of blood analyses in wild vertebrates, and proposes a standardisation of pre-analytical procedures plus some suggestions for a more systematic examination of blood smears to increase the diagnostic value of blood data. By discussing the common problems that field researchers face with blood variables, we also aim to highlight common ground enabling new researchers in the field to accurately collect blood samples and interpret and place their haematological findings into the overall picture of an ecological or eco-toxicological study. Besides showing the practicality and ecological relevance of simple blood variables, this study illustrates the suitability of blood samples for the application of cutting-edge analytical procedures for expanding the current repertoire of diagnostic tools in wildlife monitoring and ecosystem health assessment.
血液分析在野生动物的生态学、生态毒理学和兽医科学研究中具有很大的潜力,这得益于人类和家畜医学的进步。然而,野外研究人员的主要警告是,人类或家畜血液学的“规则”并不总是适用于野生动物。本综述展示了血液分析在野生脊椎动物中的优势和局限性,并提出了标准化的分析前程序,以及一些关于更系统地检查血涂片的建议,以提高血液数据的诊断价值。通过讨论野外研究人员在血液变量方面面临的常见问题,我们还旨在强调共同点,使该领域的新研究人员能够准确地采集血液样本,并将他们的血液学发现纳入生态或生态毒理学研究的整体情况。本研究除了展示简单血液变量的实用性和生态相关性外,还说明了血液样本适用于应用先进的分析程序,以扩大野生动物监测和生态系统健康评估中诊断工具的现有范围。