Kozler P, Riljak V, Jandová K, Pokorný J
Institute of Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
Physiol Res. 2014;63(Suppl 4):S529-34. doi: 10.33549/physiolres.932935.
In our previous experiments we demonstrated that osmotic opening of the blood brain barrier (BBB) in rats by administration of mannitol into the internal carotid artery leads to cerebral edema. The aim of this study was to confirm objectively the development of brain edema and determine whether it affects spontaneous locomotor activity in rats (SLA). Brain edema was verified by computer tomography (CT) examination of the brain and SLA was observed during open field test. Twenty four adult male rats were divided into four groups of six: (1) control animals (C), (2) controls with anesthesia (CA), (3) controls with sham surgery (CS), (4) experimental - osmotic opening of the BBB (MA). Osmotic BBB disruption manifested by reducing the density of brain tissue (hypodensity), suggesting a higher water content in the brain tissue. SLA was compared between C, CA, CS and MA groups and between MA and CA groups. Significant difference was found only between the control group and MA group. In the first 30 min of the examination, rats after the mannitol administration revealed a marked limitation of spontaneous locomotor activity. Experimental results demonstrated reduction of spontaneous locomotor activity in rats with induced brain edema.
在我们之前的实验中，我们证明了通过向大鼠颈内动脉注射甘露醇来渗透性开放血脑屏障（BBB）会导致脑水肿。本研究的目的是客观地证实脑水肿的发展，并确定其是否影响大鼠的自发运动活动（SLA）。通过脑部计算机断层扫描（CT）检查来验证脑水肿，并在旷场试验中观察SLA。将24只成年雄性大鼠分为四组，每组6只：（1）对照动物（C），（2）麻醉对照组（CA），（3）假手术对照组（CS），（4）实验性 - 血脑屏障渗透性开放组（MA）。血脑屏障的渗透性破坏表现为脑组织密度降低（低密度），提示脑组织含水量增加。比较了C、CA、CS和MA组之间以及MA组和CA组之间的SLA。仅在对照组和MA组之间发现了显著差异。在检查的前30分钟内，注射甘露醇后的大鼠自发运动活动明显受限。实验结果表明，诱导脑水肿的大鼠自发运动活动减少。
