Institute of Physiology, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Physiol Res. 2020 Nov 16;69(5):919-926. doi: 10.33549/physiolres.934460. Epub 2020 Sep 9.
Magnetic resonance imaging has been used for evaluating of a brain edema in experimental animals to assess cytotoxic and vasogenic edema by the apparent diffusion coefficient (ADC) and T2 imaging. This paper brings information about the effectiveness of methylprednisolone (MP) on experimental brain edema. A total of 24 rats were divided into three groups of 8 animals each. Rats with cytotoxic/intracellular brain edema induced by water intoxication were assigned to the group WI. These rats also served as the additional control group CG when measured before the induction of edema. A third group (WIMP) was intraperitoneally administered with methylprednisolone 100 mg/kg during water intoxication treatment. The group WI+MP was injected with methylprednisolone 50 mg/kg into the carotid artery within two hours after the water intoxication treatment. We evaluated the results in four groups. Two control groups (CG, WI) and two experimental groups (WIMP, WI+MP). Rats were subjected to MR scanning 24 h after edema induction. We observed significantly increased ADC values in group WI in both evaluated areas - cortex and hippocampus, which proved the occurrence of experimental vasogenic edema, while ADC values in groups WIMP and WI+MP were not increased, indicating that the experimental edema was not developed and thus confirming the protective effect of MP.
磁共振成像已被用于评估实验动物的脑水肿，通过表观扩散系数（ADC）和 T2 成像来评估细胞毒性和血管源性水肿。本文提供了关于甲基强的松龙（MP）对实验性脑水肿的有效性的信息。共有 24 只大鼠分为三组，每组 8 只。由水中毒引起细胞毒性/细胞内脑水肿的大鼠被分配到 WI 组。这些大鼠在脑水肿诱导前也作为附加对照组 CG 进行测量。第三组（WIMP）在水中毒治疗期间腹腔内给予 100mg/kg 甲基强的松龙。WI+MP 组在水中毒治疗后两小时内通过颈动脉注射 50mg/kg 甲基强的松龙。我们在四组中评估了结果。两个对照组（CG、WI）和两个实验组（WIMP、WI+MP）。大鼠在脑水肿诱导后 24 小时进行磁共振扫描。我们观察到 WI 组两个评估区域（皮质和海马体）的 ADC 值显著增加，这证明了实验性血管源性水肿的发生，而 WIMP 组和 WI+MP 组的 ADC 值没有增加，表明实验性水肿没有发展，从而证实了 MP 的保护作用。
