用于灵敏区分血浆和细胞中半胱氨酸/同型半胱氨酸及谷胱甘肽的双发射通道。
Dual emission channels for sensitive discrimination of Cys/Hcy and GSH in plasma and cells.
作者信息
Zhang Yuanlin, Shao Xiangmin, Wang Yue, Pan Fuchao, Kang Ruixue, Peng Fangfang, Huang Zhentao, Zhang Weijuan, Zhao Weili
机构信息
Key Laboratory for Special Functional Materials of the Ministry of Education, Henan University, Kaifeng, 475004, P. R. China.
出版信息
Chem Commun (Camb). 2015 Mar 11;51(20):4245-8. doi: 10.1039/c4cc08687b.
A new selective fluorescent and colorimetric chemosensor for the detection of GSH was developed. The discrimination of GSH from Cys and Hcy is achieved through two emission channel detection. The detection limit of probe 1 for GSH reached 10 nM (3 ppb). The excellent sensitivity and selectivity of probe 1 allow the selective detection of GSH over Cys and Hcy, which can be visualized colorimetrically and/or fluorescently. The sensitive detection of GSH allowed for convenient measurement of the GSH content in human plasma. The presence of GSH in cells was demonstrated through cell imaging.
开发了一种用于检测谷胱甘肽（GSH）的新型选择性荧光和比色化学传感器。通过两个发射通道检测实现了GSH与半胱氨酸（Cys）和同型半胱氨酸（Hcy）的区分。探针1对GSH的检测限达到10 nM（3 ppb）。探针1出色的灵敏度和选择性使得其能够选择性地检测GSH，而不是Cys和Hcy，并且可以通过比色法和/或荧光法进行可视化检测。GSH的灵敏检测使得方便地测量人血浆中的GSH含量成为可能。通过细胞成像证明了细胞中存在GSH。
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