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一种基于氮杂硼二吡咯的近红外荧光探针，用于灵敏区分活细胞中的半胱氨酸/同型半胱氨酸和谷胱甘肽。

An aza-BODIPY based near-infrared fluorescent probe for sensitive discrimination of cysteine/homocysteine and glutathione in living cells.

作者信息

Xiang Hui-Jing, Tham Huijun Phoebe, Nguyen Minh Duc, Fiona Phua Soo Zeng, Lim Wei Qi, Liu Jin-Gang, Zhao Yanli

机构信息

Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.

出版信息

Chem Commun (Camb). 2017 May 4;53(37):5220-5223. doi: 10.1039/c7cc01814b.

DOI:10.1039/c7cc01814b
PMID:28443883
Abstract

Discriminative detection of glutathione (GSH) from cysteine/homocysteine (Cys/Hcy) is achieved through two emission channel analysis using a stable, highly sensitive, and selective near-infrared fluorescent probe that bears 7-nitrobenzo-2-oxa-1,3-diazole and aza-BODIPY units. The probe was successfully applied for simultaneous determination of GSH and Cys/Hcy in living cells.

摘要

通过使用一种带有7-硝基苯并-2-恶唑-1,3-二唑和氮杂-BODIPY单元的稳定、高灵敏度且选择性的近红外荧光探针，利用两个发射通道分析实现了从半胱氨酸/同型半胱氨酸（Cys/Hcy）中鉴别检测谷胱甘肽（GSH）。该探针已成功应用于同时测定活细胞中的GSH和Cys/Hcy。

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