Muscatello L V, Sarli G, Beha G, Asproni P, Millanta F, Poli A, De Tolla L J, Benazzi C, Brunetti B
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 40064 Ozzano Emilia, Bologna, Italy.
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50 40064 Ozzano Emilia, Bologna, Italy.
J Comp Pathol. 2015 Feb-Apr;152(2-3):153-60. doi: 10.1016/j.jcpa.2014.12.014. Epub 2015 Feb 7.
Tissue microarray (TMA) is a high-throughput method adopted for simultaneous molecular profiling of tissue samples from large patient cohorts. The aim of this study was to validate the TMA method for the molecular classification of canine and feline mammary tumours. Twelve samples, five feline and five canine mammary tumours and two canine haemangiosarcomas, were collected. TMA construction was based on Kononen's method of extracting a cylindrical core of paraffin wax-embedded 'donor' tissue and inserting it into a 'recipient' wax block. Seven consecutive sections from each tissue array block were subjected to immunohistochemistry (IHC) using primary antibodies specific for oestrogen receptor (OR), progesterone receptor (PR), c-erbB-2, cytokeratin (CK) 5/6, CK14, CK19 and p63. The same panel of antibodies was applied to the full sections from all cases. Comparison between full sections and TMA scores revealed different results depending on the antibodies. Labelling for OR, PR, CK19 and p63 showed total concordance, c-erbB2 (score +2, +3) was concordant in nine out of ten cases, CK5/6 and CK14 in eight out of ten cases. The TMA platform preserves the molecular profile of canine and feline mammary tumour markers, representing a useful tool for rapid and cost-effective analysis for the first phenotypic screening using OR, PR and c-erbB2 antibodies. Basal cytokeratin, used for triple negative identification, shows a multifocal 'niche' expression pattern, for which IHC of the full section or multiple core array is recommended.
组织微阵列（TMA）是一种高通量方法，用于对来自大量患者队列的组织样本进行同步分子分析。本研究的目的是验证TMA方法在犬猫乳腺肿瘤分子分类中的应用。收集了12个样本，包括5个猫乳腺肿瘤、5个犬乳腺肿瘤和2个犬血管肉瘤。TMA构建基于Kononen的方法，即从石蜡包埋的“供体”组织中提取圆柱形蜡芯，并将其插入“受体”蜡块中。对每个组织阵列块的连续7个切片进行免疫组织化学（IHC）检测，使用针对雌激素受体（OR）、孕激素受体（PR）、c-erbB-2、细胞角蛋白（CK）5/6、CK14、CK19和p63的一抗。将同一组抗体应用于所有病例的完整切片。完整切片和TMA评分之间的比较显示，根据抗体的不同结果也不同。OR、PR、CK19和p63的标记显示完全一致，c-erbB2（评分+2、+3）在十分之九的病例中一致，CK5/6和CK14在十分之八的病例中一致。TMA平台保留了犬猫乳腺肿瘤标志物的分子特征，是使用OR、PR和c-erbB2抗体进行首次表型筛选的快速且经济高效分析的有用工具。用于三阴性鉴定的基底细胞角蛋白显示出多灶性“小生境”表达模式，建议对完整切片或多核阵列进行IHC检测。
