Bottomly K, Luqman M, Greenbaum L, Carding S, West J, Pasqualini T, Murphy D B
Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510.
Eur J Immunol. 1989 Apr;19(4):617-23. doi: 10.1002/eji.1830190407.
CD4 T cell clones have been shown to be functionally heterogeneous in the mouse. However, it is not known if normal CD4 T cells are also functionally heterogeneous, or whether functional specialization is a result of cloning and long-term culture. To approach this question, a monoclonal antibody reacting with a subset of CD4 T cells has been prepared by immunization of rats with different cloned T cell lines all sharing the same functional activity. This monoclonal antibody reacts with a subset of CD45 (T200) molecules by binding to a determinant requiring the expression of the second variable exon of the CD45 molecule. Some CD4 T cells bear high levels of this marker, while others react only weakly. This antibody was used to separate CD4 T cells into two subpopulations. The brightly staining population was found to produce interleukin (IL) 2 and not IL 4, while the weakly staining population produced IL 4 and not IL 2. These data demonstrate that CD4 T cells in normal mice are already functionally committed, and that they differentially express forms of CD45 that contain the second variable exon.
在小鼠中，CD4 T细胞克隆已显示出功能上的异质性。然而，尚不清楚正常的CD4 T细胞是否也存在功能异质性，或者功能特化是否是克隆和长期培养的结果。为了解决这个问题，通过用具有相同功能活性的不同克隆T细胞系免疫大鼠，制备了一种与一部分CD4 T细胞反应的单克隆抗体。这种单克隆抗体通过结合需要CD45分子第二个可变外显子表达的决定簇，与一部分CD45（T200）分子发生反应。一些CD4 T细胞带有高水平的这种标志物，而其他细胞的反应则很弱。该抗体用于将CD4 T细胞分为两个亚群。发现染色明亮的群体产生白细胞介素（IL）-2而不产生IL-4，而染色较弱的群体产生IL-4而不产生IL-2。这些数据表明，正常小鼠中的CD4 T细胞在功能上已经定向分化，并且它们差异表达含有第二个可变外显子的CD45形式。
