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基于线粒体标记细胞色素氧化酶I对茄子蛀茎蛀果蛾(Leucinodes orbonalis (Guenée),鳞翅目:草螟科)的分子特征分析及其系统发育关系

Molecular characterization of brinjal shoot and fruit borer, Leucinodes orbonalis (Guenée) (Lepidoptera: Crambidae) based on mitochondrial marker cytochrome oxidase I and their phylogenetic relationship.

作者信息

Shashank P R, Ojha Rakshit, Venkatesan T, Jalali S K, Bhanu K R M

出版信息

Indian J Exp Biol. 2015 Jan;53(1):51-5.

Abstract

Shoot and fruit borer, Leucinodes orbonalis is an important insect pest infesting brinjal or eggplant in India. Molecular characterization of nine different populations belonging to various brinjal growing regions was done using Cytochorome C Oxidase I (COI) gene. Nucleotide analysis of genetic diversity and phylogenetic analysis of the COI indicate that the L. orbonalis from different geographical regions are homogenous. The results showed less nucleotide diversity (π = 0.007895) and overall mean distance (0.008 ± 0.003). Topologies of neighbour-joining (NJ) trees indicate all the populations belong to single major clade. Therefore, it is inferred that there was no significant molecular diversity within L. orbonalis of different geographical locations of India with respect to COI.

摘要

茄黄斑螟是印度茄子上的一种重要害虫。利用细胞色素C氧化酶I(COI)基因对来自不同茄子种植区的九个不同种群进行了分子特征分析。对COI的遗传多样性进行核苷酸分析和系统发育分析表明,来自不同地理区域的茄黄斑螟是同质的。结果显示核苷酸多样性较低(π = 0.007895),总体平均距离为(0.008 ± 0.003)。邻接法(NJ)树的拓扑结构表明所有种群都属于单一的主要进化枝。因此,可以推断,就COI而言,印度不同地理位置的茄黄斑螟之间没有显著的分子多样性。

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