Prodhan Md Zulfikar Haider, Shirale Dattatray K, Islam Md Zaherul, Hossain Md Jahangir, Paranjape Vijay, Shelton Anthony M
Tuber Crops Research Sub Centre, BARI, Bogura 5801, Bangladesh.
Mahyco Research Center, Maharashtra Hybrid Seed Company Pvt. Ltd. (Mahyco), Post Box No 76, Jalna 431213, India.
Insects. 2019 Jul 5;10(7):198. doi: 10.3390/insects10070198.
Eggplant ( Linn.), or brinjal, was engineered to express an insecticidal protein (Cry1Ac) from (Bt) and commercialized in Bangladesh on a limited basis in 2014. As part of an insect resistance management strategy, studies were conducted to determine the susceptibility of the targeted insect pest, the eggplant fruit and shoot borer, (Guenée), to Cry1Ac using a diet-incorporation bioassay method. Eighteen populations of were collected from the main brinjal growing areas in 17 districts of Bangladesh during 2018-2019 and assayed. Larvae from each population were reared to adults and allowed to mate. Eggs from the matings were allowed to hatch, and neonates were used for bioassays. Bioassays were performed with different concentrations of Cry1Ac and an untreated control. Median lethal concentrations (LC) ranged between 0.035 and 0.358 ppm and molt inhibitory concentration (MIC) values ranged from 0.008 to 0.181 ppm. Variation in susceptibility among field populations was 10.22-fold for LC and 22.63-fold for MIC. These results were compared to values from 73 populations in India. Overall, the results showed similar natural variation and suggest that these Bangladeshi values can be used as benchmarks for resistance monitoring as Bt brinjal becomes more widely adopted in Bangladesh.
茄子（茄属），即茄子，被改造以表达来自苏云金芽孢杆菌（Bt）的一种杀虫蛋白（Cry1Ac），并于2014年在孟加拉国有限度地商业化种植。作为抗虫管理策略的一部分，开展了研究以使用饲料掺入生物测定法来确定目标害虫茄子果实和嫩茎螟蛾（Guenée）对Cry1Ac的敏感性。2018 - 2019年期间从孟加拉国17个地区的主要茄子种植区收集了18个茄子果实和嫩茎螟蛾种群并进行了测定。每个种群的幼虫饲养至成虫并使其交配。交配产生的卵孵化后，将新生幼虫用于生物测定。生物测定使用不同浓度的Cry1Ac和一个未处理对照进行。半数致死浓度（LC）在0.035至0.358 ppm之间，蜕皮抑制浓度（MIC）值在0.008至0.181 ppm之间。田间种群之间的敏感性差异对于LC为10.22倍，对于MIC为22.63倍。这些结果与印度73个种群的值进行了比较。总体而言，结果显示出相似的自然变异，并表明随着Bt茄子在孟加拉国更广泛地被采用，这些孟加拉国的值可作为抗性监测的基准。
