Sharma Shashi, Saxena Sandeep, Srivastav Khushboo, Shukla Rajendra K, Mishra Nibha, Meyer Carsten H, Kruzliak Peter, Khanna Vinay K
Retina Service, Department of Ophthalmology, King George's Medical University, Lucknow, India.
Developmental Toxicology Division, Indian Institute of Toxicology and Research, Lucknow, India.
Clin Exp Ophthalmol. 2015 Jul;43(5):429-36. doi: 10.1111/ceo.12506. Epub 2015 Jun 26.
The aim of the study was to determine plasma nitric oxide (NO) and lipid peroxide (LPO) levels in diabetic retinopathy and its association with severity of disease.
Prospective observational study.
A total of 60 consecutive cases and 20 healthy controls were included.
Severity of retinopathy was graded according to early treatment diabetic retinopathy study (ETDRS) classification. Photoreceptor inner segment ellipsoid band (ISel) disruption and retinal pigment epithelium (RPE) alteration were graded using spectral domain optical coherence tomography. Data were statistically analyzed.
Plasma thiobarbituric acid reactive substances, NO assay and reduced glutathione (GSH) were measured using standard protocol.
Increased severity of diabetic retinopathy was significantly associated with increase in plasma levels of LPO (P < 0.05), NO (P < 0.001) and decrease in plasma levels of GSH (P < 0.0001), ISel disruption (P < 0.001) and RPE topographic alteration (P < 0.01).
Increased plasma NO levels are associated with increased severity of diabetic retinopathy. For the first time, it has been demonstrated that increased plasma LPO, NO and decreased GSH levels are associated with in vivo structural changes in inner segment ellipsoid and RPE.
本研究旨在测定糖尿病视网膜病变患者的血浆一氧化氮（NO）和脂质过氧化物（LPO）水平，并探讨其与疾病严重程度的关系。
前瞻性观察性研究。
共纳入60例连续病例和20名健康对照者。
根据早期糖尿病视网膜病变研究（ETDRS）分类法对视网膜病变的严重程度进行分级。使用光谱域光学相干断层扫描对光感受器内节椭圆体带（ISel）破坏和视网膜色素上皮（RPE）改变进行分级。对数据进行统计学分析。
采用标准方案测定血浆硫代巴比妥酸反应性物质、NO含量及还原型谷胱甘肽（GSH）水平。
糖尿病视网膜病变严重程度增加与血浆LPO水平升高（P < 0.05）、NO水平升高（P < 0.001）、GSH水平降低（P < 0.0001）、ISel破坏（P < 0.001）以及RPE地形改变（P < 0.01）显著相关。
血浆NO水平升高与糖尿病视网膜病变严重程度增加有关。首次证明血浆LPO、NO水平升高及GSH水平降低与内节椭圆体和RPE的体内结构变化有关。
