Liu Shanshan, Yu Xiongwei, Hu Baoji, Zou Yun, Li Jinbao, Bo Lulong, Deng Xiaoming
Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, Shanghai, China; Department of Anesthesiology, Chenggong Hospital, Xiamen University, Xiamen, China.
Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, Shanghai, China; Department of Anesthesiology, 285th Hospital of CPLA, Handan, China.
J Surg Res. 2015 May 1;195(1):277-83. doi: 10.1016/j.jss.2015.01.021. Epub 2015 Jan 14.
Salidroside (SDS) is the main effective component of Rhodiola rosea L with a variety of pharmacologic properties. The objective of this study was to investigate the efficacy of SDS in the treatment of experimental sepsis in mice and explore the possible underlying action mechanisms.
Sepsis was induced in C57BL/6 male mice via cecal ligation and puncture (CLP). The animals were divided into three groups as follows: sham, CLP, and CLP plus SDS. SDS (50 mg/kg) was injected intraperitoneally 1 h after operation. Postoperative survival of the mice, bacterial clearance in blood and peritoneal lavage fluid, cytokine secretion in blood, and histology of lung were evaluated. In addition, apoptosis of immune cells in the spleen and thymus were examined, respectively.
SDS administration prolonged the survival of the septic mice, inhibited the proinflammatory responses, and enhanced bacterial clearance. It also alleviated the pathologic changes in the lung and inhibited the apoptosis of immune cells in the spleen and thymus after CLP challenge.
SDS exerts a protective effect in CLP-induced sepsis by attenuating the proinflammatory responses, enhancing bacterial clearance, and preserving adaptive immunity. SDS may be a promising therapeutic strategy for the treatment of sepsis.
红景天苷（SDS）是红景天的主要有效成分，具有多种药理特性。本研究的目的是探讨SDS对小鼠实验性脓毒症的治疗效果，并探索其可能的潜在作用机制。
通过盲肠结扎和穿刺（CLP）诱导C57BL/6雄性小鼠发生脓毒症。将动物分为三组：假手术组、CLP组和CLP加SDS组。术后1小时腹腔注射SDS（50mg/kg）。评估小鼠术后存活率、血液和腹腔灌洗液中的细菌清除率、血液中的细胞因子分泌以及肺组织学。此外，分别检测脾脏和胸腺中免疫细胞的凋亡情况。
给予SDS可延长脓毒症小鼠的存活时间，抑制促炎反应，增强细菌清除能力。它还减轻了CLP攻击后肺组织的病理变化，并抑制了脾脏和胸腺中免疫细胞的凋亡。
SDS通过减轻促炎反应、增强细菌清除能力和维持适应性免疫，对CLP诱导的脓毒症发挥保护作用。SDS可能是一种有前途的脓毒症治疗策略。
