Salidroside promotes human periodontal ligament cell proliferation and osteocalcin secretion via ERK1/2 and PI3K/Akt signaling pathways.

Salidroside modulates cell proliferation and serves as an anti-inflammatory and anti-apoptotic agent with efficacy against various diseases. The objective of the present study was to investigate the efficacy of salidroside in enhancing the proliferation of human periodontal ligament cells (hPDLCs). hPDLCs were isolated and the effects of salidroside on cell viability, soluble osteocalcin levels and activation of proliferation-associated signaling pathways were determined using a CCK-8 assay, ELISA and Western blotting, respectively. The results indicated that salidroside induced proliferation of hPDLCs, increased secretion of soluble osteocalcin and enhanced activation of extracellular signal-regulated kinase (ERK)1/2 and phosphoinositide-3 kinase (PI3K)/Akt signaling pathways. These factors were upregulated by salidroside in a dose-dependent manner. The results of the present study suggested that salidroside mediated hPDLC proliferation via the ERK1/2 and PI3K/Akt signaling pathways, as well as osteocalcin secretion. Salidroside may therefore be used as a novel therapeutic agent in the treatment of the tooth-supporting apparatus, progressive tooth destruction or periodontitis.