Zhao Xinjie, Zhou Lina, Yin Peiyuan, Xu Guowang
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, 16023, Dalian, China.
Methods Mol Biol. 2015;1277:61-73. doi: 10.1007/978-1-4939-2377-9_6.
Metabonomics aims at a comprehensive and semiquantitative monitoring of the perturbations of the metabonome in response to pathophysiological stimuli. Liquid chromatography-mass spectrometry (LC-MS) is one of the most commonly employed analytical platforms for this purpose. As the sample pretreatment is detailed in the former chapter, we here describe the practical procedures for the LC-MS-based metabolic profiling of biological samples including detailed liquid chromatographic and MS conditions, batch analysis, peak alignment, data quality assessment, and compound identification.
代谢组学旨在全面且半定量地监测代谢组在病理生理刺激下的扰动情况。液相色谱-质谱联用（LC-MS）是为此目的最常用的分析平台之一。由于前一章已详细介绍了样品预处理，我们在此描述基于LC-MS的生物样品代谢谱分析的实际操作步骤，包括详细的液相色谱和质谱条件、批次分析、峰对齐、数据质量评估以及化合物鉴定。
