Emwas Abdul-Hamid M, Al-Talla Zeyad A, Kharbatia Najeh M
NMR Core Lab, King Abdullah University of Science and Technology, Room 0149, 23955-6900, Thuwal, Kingdom of Saudi Arabia,
Methods Mol Biol. 2015;1277:75-90. doi: 10.1007/978-1-4939-2377-9_7.
To maximize the utility of gas chromatography-mass spectrometry (GC-MS) in metabonomics research, all stages of the experimental design should be standardized, including sample collection, storage, preparation, and sample separation. Moreover, the prerequisite for any GC-MS analysis is that a compound must be volatile and thermally stable if it is to be analyzed using this technique. Since many metabolites are nonvolatile and polar in nature, they are not readily amenable to analysis by GC-MS and require initial chemical derivatization of the polar functional groups in order to reduce the polarity and to increase the thermal stability and volatility of the analytes. In this chapter, an overview is presented of the optimum approach to sample collection, storage, and preparation for gas chromatography-mass spectrometry-based metabonomics with particular focus on urine samples as example of biofluids.
为了在代谢组学研究中最大限度地发挥气相色谱 - 质谱联用（GC-MS）的效用，实验设计的各个阶段都应标准化，包括样品采集、储存、制备和样品分离。此外，任何GC-MS分析的前提条件是，如果要使用该技术进行分析，化合物必须具有挥发性且热稳定。由于许多代谢物本质上是不挥发且极性的，它们不易通过GC-MS进行分析，需要对极性官能团进行初步化学衍生化，以降低极性并提高分析物的热稳定性和挥发性。在本章中，将概述基于气相色谱 - 质谱联用的代谢组学的样品采集、储存和制备的最佳方法，特别以尿液样本作为生物流体的示例进行重点介绍。
